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Modulation of Differentiation Processes in Murine Embryonic Stem Cells Exposed to Parabolic Flight-Induced Acute Hypergravity and Microgravity

hal.structure.identifierCenter for Molecular Medicine [Cologne] [CMMC]
dc.contributor.authorACHARYA, Aviseka
hal.structure.identifierGerman Aerospace Center [DLR]
dc.contributor.authorBRUNGS, Sonja
hal.structure.identifierCenter for Molecular Medicine [Cologne] [CMMC]
dc.contributor.authorHENRY, Margit
hal.structure.identifierCenter for Molecular Medicine [Cologne] [CMMC]
dc.contributor.authorROTSHTEYN, Tamara
hal.structure.identifierCenter for Molecular Medicine [Cologne] [CMMC]
dc.contributor.authorSINGH YADUVANSHI, Nirmala
hal.structure.identifierGerman Aerospace Center [DLR]
dc.contributor.authorWEGENER, Lucia
hal.structure.identifierGerman Aerospace Center [DLR]
dc.contributor.authorJENTZSCH, Simon
hal.structure.identifierCenter for Molecular Medicine [Cologne] [CMMC]
dc.contributor.authorHESCHELER, Jürgen
hal.structure.identifierGerman Aerospace Center [DLR]
dc.contributor.authorHEMMERSBACH, Ruth
hal.structure.identifierBioingénierie tissulaire [BIOTIS]
dc.contributor.authorBOEUF, Hélène
ORCID: 0000-0002-3006-8773
IDREF: 03205453X
hal.structure.identifierCenter for Molecular Medicine [Cologne] [CMMC]
dc.contributor.authorSACHINIDIS, Agapios
dc.date.accessioned2021-06-10T07:03:42Z
dc.date.available2021-06-10T07:03:42Z
dc.date.issued2018-06-15
dc.identifier.issn1547-3287
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/78930
dc.description.abstractEnEmbryonic developmental studies under microgravity conditions in space are very limited. To study the effects of short-term altered gravity on embryonic development processes, we exposed mouse embryonic stem cells (mESCs) to phases of hypergravity and microgravity and studied the differentiation potential of the cells using wide-genome microarray analysis. During the 64th European Space Agency's parabolic flight campaign, mESCs were exposed to 31 parabolas. Each parabola comprised phases lasting 22 s of hypergravity, microgravity, and a repeat of hypergravity. On different parabolas, RNA was isolated for microarray analysis. After exposure to 31 parabolas, mESCs (P31 mESCs) were further differentiated under normal gravity (1 g) conditions for 12 days, producing P31 12-day embryoid bodies (EBs). After analysis of the microarrays, the differentially expressed genes were analyzed using different bioinformatic tools to identify developmental and nondevelopmental biological processes affected by conditions on the parabolic flight experiment. Our results demonstrated that several genes belonging to GOs associated with cell cycle and proliferation were downregulated in undifferentiated mESCs exposed to gravity changes. However, several genes belonging to developmental processes, such as vasculature development, kidney development, skin development, and to the TGF-β signaling pathway, were upregulated. Interestingly, similar enriched and suppressed GOs were obtained in P31 12-day EBs compared with ground control 12-day EBs. Our results show that undifferentiated mESCs exposed to alternate hypergravity and microgravity phases expressed several genes associated with developmental/differentiation and cell cycle processes, suggesting a transition from the undifferentiated pluripotent to a more differentiated stage of mESCs.
dc.language.isoen
dc.publisherMary Ann Liebert
dc.subject.entranscriptomics.
dc.subject.endifferentiation
dc.subject.enembryonic stem cells
dc.subject.enhypergravity
dc.subject.enmicrogravity
dc.subject.enparabolic flight
dc.title.enModulation of Differentiation Processes in Murine Embryonic Stem Cells Exposed to Parabolic Flight-Induced Acute Hypergravity and Microgravity
dc.typeArticle de revue
dc.identifier.doi10.1089/scd.2017.0294
dc.subject.halSciences du Vivant [q-bio]
bordeaux.journalStem Cells and Development
bordeaux.page838-847
bordeaux.volume27
bordeaux.hal.laboratoriesBioingénierie Tissulaire (BioTis) - U1026*
bordeaux.issue12
bordeaux.institutionCNRS
bordeaux.institutionINSERM
bordeaux.institutionCHU de Bordeaux
bordeaux.institutionInstitut Bergonié
bordeaux.peerReviewedoui
hal.identifierinserm-02870976
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//inserm-02870976v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Stem%20Cells%20and%20Development&rft.date=2018-06-15&rft.volume=27&rft.issue=12&rft.spage=838-847&rft.epage=838-847&rft.eissn=1547-3287&rft.issn=1547-3287&rft.au=ACHARYA,%20Aviseka&BRUNGS,%20Sonja&HENRY,%20Margit&ROTSHTEYN,%20Tamara&SINGH%20YADUVANSHI,%20Nirmala&rft.genre=article


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