PAIVA DOS SANTOS, Bruno; GARBAY, Bertrand; PASQUA, Mattia; CHEVRON, Elsa; CHINOY, Zoeisha S.; CULLIN, Christophe; BATHANY, Katell; LECOMMANDOUX, Sebastien; AMEDEE, Joelle; OLIVEIRA, Hugo; GARANGER, Elisabeth

doi:10.1016/j.jbiotec.2019.04.010

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PAIVA DOS SANTOS, Bruno

Bioingénierie tissulaire [BIOTIS]

GARBAY, Bertrand

Laboratoire de Chimie des Polymères Organiques [LCPO]

PASQUA, Mattia
Bioingénierie tissulaire [BIOTIS]

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Article de revue

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Journal of Biotechnology. 2019, vol. 298, p. 35-44

Résumé en anglais

Elastin-like polypeptides (ELPs) are biocompatible-engineered polypeptides, with promising interest in tissue engineering due to their intrinsic biological and physical properties, and their ease of production. The IKVAV (Ile-Lys-Val-Ala-Val) laminin-1 sequence has been shown to sustain neuron attachment and growth. In this study, the IKVAV adhesion sequence, or a scrambled VKAIV sequence, were incorporated by genetic engineering in the structure of an ELP, expressed in Escherichia coli and purified. The transition temperatures of the ELP-IKVAV and ELP-VKAIV were determined to be 23°C. Although the phase transition was fully reversible for ELP-VKAIV, we observed an irreversible aggregation for ELP-IKVAV. The corresponding aggregates shared some characteristics with amyloid-like polypeptides. The two ELPs were then reacted with functionalized polyethylene glycol (PEG) to form hydrogels. These hydrogels were characterized for rheological properties, tested with cultures of rat primary sensory neurons, and implanted subcutaneously in mice for 4 weeks. Sensory neurons cultured on high IKVAV concentration hydrogels (20%) formed longer neurite than those of neurons grown on hydrogels containing the scrambled IKVAV sequence. Finally, in vivo evaluation showed the absence of detectable inflammation. In conclusion, this functionalized ELP-IKVAV biomaterial shows interesting properties for tissue engineering requiring neurotization.< Réduire

Mots clés en anglais

Elastin-like polypeptides

Recombinant expression

Hydrogels

Sensory neurons

Tissue-engineering

URI

https://oskar-bordeaux.fr/handle/20.500.12278/7514

DOI

http://dx.doi.org/10.1016/j.jbiotec.2019.04.010

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Production, purification and characterization of an elastin-like polypeptide containing the Ile-Lys-Val-Ala-Val (IKVAV) peptide for tissue engineering applications

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Production, purification and characterization of an elastin-like polypeptide containing the Ile-Lys-Val-Ala-Val (IKVAV) peptide for tissue engineering applications

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Résumé en anglais

Mots clés en anglais

URI

DOI

Unités de recherche