The Bacterial Amyloid-Like Hfq Promotes In Vitro DNA Alignment
| dc.rights.license | open | en_US |
| dc.contributor.author | WIEN, Frank | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | MARTINEZ, Denis | |
| dc.contributor.author | LE BRUN, Etienne | |
| dc.contributor.author | JONES, Nykola C. | |
| dc.contributor.author | VRONNING HOFFMANN, Soren | |
| dc.contributor.author | WAEYTENS, Jehan | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | BERBON, Melanie | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | HABENSTEIN, Birgit | |
| dc.contributor.author | ARLUISON, Veronique | |
| dc.date.accessioned | 2020-04-22T08:22:27Z | |
| dc.date.available | 2020-04-22T08:22:27Z | |
| dc.date.issued | 2019 | |
| dc.identifier.issn | 2076-2607 | en_US |
| dc.identifier.other | https://www.mdpi.com/2076-2607/7/12/639/s1 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/4330 | |
| dc.description.abstractEn | The Hfq protein is reported to be involved in environmental adaptation and virulence of several bacteria. In Gram-negative bacteria, Hfq mediates the interaction between regulatory noncoding RNAs and their target mRNAs. Besides these RNA-related functions, Hfq is also associated with DNA and is a part of the bacterial chromatin. Its precise role in DNA structuration is, however, unclear and whether Hfq plays a direct role in DNA-related processes such as replication or recombination is controversial. In previous works, we showed that Escherichia coli Hfq, or more precisely its amyloid-like C-terminal region (CTR), induces DNA compaction into a condensed form. In this paper, we evidence a new property for Hfq; precisely we show that its CTR influences double helix structure and base tilting, resulting in a strong local alignment of nucleoprotein Hfq:DNA fibers. The significance of this alignment is discussed in terms of chromatin structuration and possible functional consequences on evolutionary processes and adaptation to environment. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | Nucleoid-associated protein (NAP) | |
| dc.subject.en | DNA compaction; DNA recombination | |
| dc.subject.en | amyloid; Sm protein; Synchrotron Radiation Circular and Linear Dichroism (SRCD/SRLD); Couette flow cell | |
| dc.subject.en | atomic force microscopy (AFM) | |
| dc.subject.en | solid state NMR (ssNMR) | |
| dc.title.en | The Bacterial Amyloid-Like Hfq Promotes In Vitro DNA Alignment | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.3390/microorganisms7120639 | en_US |
| dc.subject.hal | Chimie/Matériaux | en_US |
| bordeaux.journal | Microorganisms | en_US |
| bordeaux.volume | 7 | en_US |
| bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248 | en_US |
| bordeaux.issue | 12 | en_US |
| bordeaux.institution | Bordeaux INP | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.identifier | hal-02550350 | |
| hal.version | 1 | |
| hal.date.transferred | 2020-04-22T08:22:31Z | |
| hal.export | true | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Microorganisms&rft.date=2019&rft.volume=7&rft.issue=12&rft.eissn=2076-2607&rft.issn=2076-2607&rft.au=WIEN,%20Frank&MARTINEZ,%20Denis&LE%20BRUN,%20Etienne&JONES,%20Nykola%20C.&VRONNING%20HOFFMANN,%20Soren&rft.genre=article |
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