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The spatial patterning of RGD and BMP-2 mimetic peptides at the subcellular scale modulates human mesenchymal stem cells osteogenesis

dc.rights.licenseopenen_US
dc.contributor.authorBILEM, Ibrahim
hal.structure.identifierCentre d'Etudes Nucléaires de Bordeaux Gradignan [CENBG]
dc.contributor.authorPLAWINSKI, Laurent
dc.contributor.authorCHEVALLIER, Pascale
hal.structure.identifierLaboratoire de l'intégration, du matériau au système [IMS]
dc.contributor.authorAYELA, Cedric
IDREF: 125917287
dc.contributor.authorSONE, Eli D.
dc.contributor.authorLAROCHE, Gaetan
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorDURRIEU, Marie-Christine
dc.date.accessioned2020-04-17T09:34:47Z
dc.date.available2020-04-17T09:34:47Z
dc.date.issued2018
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/4293
dc.description.abstractEnEngineering artificial extracellular matrices, based on the biomimicry of the spatial distribution of proteins and growth factors within their native microenvironment, is of great importance for understanding mechanisms of bone tissue regeneration. Herein, photolithography is used to decorate glass surfaces with subcellular patterns of RGD and BMP-2 ligands; two mimetic peptides recognized to be involved in stem cells osteogenesis. The biological relevance of well-defined RGD and BMP-2 patterned surfaces is evaluated by investigating the differentiation of human mesenchymal stem cells (hMSCs) into osteoblasts, in the absence of induction media. The extent of hMSCs differentiation is revealed to be dependent on both the pattern shape and the ligand type. Indeed, the spatial patterning of BMP-2, but not RGD peptide, significantly enhances the extent of hMSCs differentiation, suggesting that geometric cues guide stem cells specification into specialized cells in a ligand type dependent manner. Such cell culture models provide an interesting tool to investigate how stem cells perceive and respond to their microenvironment and may contribute to the development of next-generation biomaterials capable of producing clinically relevant volume of bone tissue.
dc.language.isoENen_US
dc.subject.enmicropatterning
dc.subject.enmimetic peptides
dc.subject.enBMP‐2
dc.subject.enstem cell differentiation
dc.subject.enbone tissue engineering
dc.title.enThe spatial patterning of RGD and BMP-2 mimetic peptides at the subcellular scale modulates human mesenchymal stem cells osteogenesis
dc.typeArticle de revueen_US
dc.identifier.doi10.1002/jbm.a.36296
dc.subject.halChimie/Matériauxen_US
bordeaux.journalJournal of Biomedical Materials Research Part Aen_US
bordeaux.page959-970en_US
bordeaux.volume106en_US
bordeaux.hal.laboratoriesLaboratoire d’Intégration du Matériau au Système (IMS) - UMR 5218en_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.identifier.funderIDAgence Nationale de la Rechercheen_US
hal.identifierhal-03184447
hal.version1
hal.date.transferred2021-03-29T12:45:48Z
hal.exporttrue
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