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Single lipoaminoglycoside promotes efficient intracellular antibody delivery: A comprehensive insight into the mechanism of action

dc.rights.licenseopenen_US
atmire.cua.enabled10.1016/j.nano.2017.09.005
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorDALLET, Laurence
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorDECOSSAS, Marion
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorTAVEAU, Jean-Christophe
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLECOMTE, Sophie
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorPOUSSARD, Sylvie
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLAMBERT, Olivier
dc.contributor.authorPITARD, Bruno
dc.date.accessioned2020-04-15T12:37:47Z
dc.date.available2020-04-15T12:37:47Z
dc.date.issued2018
dc.identifier.issn1549-9634en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/4281
dc.description.abstractEnDelivery of biologically active proteins into cells is emerging as important strategy for many applications. Previous experiments have shown that lipoaminoglycosides were capable of delivery of the anti-cytokeratin8 antibody (anti-K8) but only when formulated with lipid helpers potentially leading to toxicity from excess lipids. Here, we optimized anti-K8 delivery with various lipoaminoglycosides in the absence of a lipid helper. Results led to the identification of the aminoglycoside lipid dioleyl phosphoramido ribostamycin (DOPRI) as a potent intracellular delivery system for anti-K8. Electron microscopy revealed that delivered anti-K8 molecules were bound to intermediate filaments in cells. Anti-K8 was bound to the surface of DOPRI vesicles without perturbing lipid organization. Macropinocytosis and caveolin mediated endocytosis contributed to anti-K8 internalization and to filament labeling with a major contribution being made by the caveolin pathway. The results showed that the unique properties of DOPRI were sufficient for efficient intracellular protein delivery without requiring lipid helpers. (C) 2017 Elsevier Inc. All rights reserved.
dc.language.isoENen_US
dc.subject.enAntibodies
dc.subject.enLipoaminoglycosides
dc.subject.enIntracellular delivery
dc.subject.enSupramolecular structure
dc.title.enSingle lipoaminoglycoside promotes efficient intracellular antibody delivery: A comprehensive insight into the mechanism of action
dc.typeArticle de revueen_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalNanomedicine-Nanotechnology Biology and Medicineen_US
bordeaux.page141-151en_US
bordeaux.volume14en_US
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248
bordeaux.issue1en_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-03184449
hal.version1
hal.date.transferred2021-03-29T12:46:58Z
hal.exporttrue
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