Assignment of H-1, C-13 and N-15 resonances and secondary structure of the Rgd1-RhoGAP domain
| dc.rights.license | open | en_US |
| dc.relation.isnodouble | 5fc053a7-6f19-4d78-946e-031242c4f98f | * |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | MARTINEZ, Denis | |
| dc.contributor.author | PROUZET-MAULEON, Valerie | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | HUGUES, Michel | |
| hal.structure.identifier | Laboratoire de biogenèse membranaire [LBM] | |
| dc.contributor.author | DOIGNON, Francois
ORCID: 0000-0002-9007-2408 IDREF: 097088439 | |
| hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
| dc.contributor.author | ODAERT, Benoit | |
| dc.date.accessioned | 2020-04-08T13:15:24Z | |
| dc.date.available | 2020-04-08T13:15:24Z | |
| dc.date.issued | 2018 | |
| dc.identifier.issn | 1874-2718 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/4183 | |
| dc.description.abstractEn | The protein Rgd1 is involved in the regulation of cytoskeleton formation and in signalling pathways that control cell polarity and growth in Saccharomyces cerevisiae. Rgd1p is composed of a F-BAR domain required for membrane binding and a RhoGAP domain responsible for activating Rho3p and Rho4p, two GTPases respectively involved in bud growth and cytokinesis. Rgd1p is recruited to the membrane through interactions with phosphoinositide lipids, which bind the two isolated domains and stimulate the RhoGAP activity on Rho4p. As previously shown by crystallography, the membrane-binding F-BAR domain contains a conserved inositol phosphate binding site, which explains the preferential binding of phosphoinositides. In contrast, RhoGAP domains are not expected to bind lipids. In order to unravel this puzzling feature, we solved the three-dimensional structure of the isolated protein and found a cryptic phosphoinositide binding site involving non conserved residues (Martinez et al. 2017). The assignment of the resonances and secondary structure of Rgd1-RhoGAP (aa 450-666) is presented here. | |
| dc.language.iso | EN | en_US |
| dc.subject.en | RhoGAP | |
| dc.subject.en | Rgd1 | |
| dc.subject.en | Assignment | |
| dc.subject.en | NMR | |
| dc.subject.en | Phosphoinositide | |
| dc.title.en | Assignment of H-1, C-13 and N-15 resonances and secondary structure of the Rgd1-RhoGAP domain | |
| dc.title.alternative | Biomol NMR Assign | en_US |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1007/s12104-017-9794-z | en_US |
| dc.subject.hal | Chimie/Matériaux | en_US |
| bordeaux.journal | Biomolecular Nmr Assignments | en_US |
| bordeaux.page | 129-132 | en_US |
| bordeaux.volume | 12 | en_US |
| bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248 | |
| bordeaux.issue | 1 | en_US |
| bordeaux.institution | Bordeaux INP | en_US |
| bordeaux.institution | Université de Bordeaux | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| hal.identifier | hal-02537007 | |
| hal.version | 1 | |
| hal.date.transferred | 2020-04-08T13:15:29Z | |
| hal.export | true | |
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