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Detection of side-chain proton resonances of fully protonated biosolids in nano-litre volumes by magic angle spinning solid-state NMR

dc.rights.licenseopenen_US
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorTOLCHARD, James
dc.contributor.authorPANDEY, Manoj Kumar
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorBERBON, Melanie
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorNOUBHANI, Abdelmajid
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorSAUPE, Sven
dc.contributor.authorNISHIYAMA, Yusuke
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorHABENSTEIN, Birgit
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorLOQUET, Antoine
dc.date.accessioned2020-04-08T10:11:47Z
dc.date.available2020-04-08T10:11:47Z
dc.date.created2018
dc.date.issued2018
dc.identifier.issn0925-2738en_US
dc.identifier.otherhttps://static-content.springer.com/esm/art%3A10.1007%2Fs10858-018-0168-3/MediaObjects/10858_2018_168_MOESM1_ESM.docxen_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/4168
dc.description.abstractEnWe present a new solid-state NMR proton-detected three-dimensional experiment dedicated to the observation of protein proton side chain resonances in nano-liter volumes. The experiment takes advantage of very fast magic angle spinning and double quantum 13C-13C transfer to establish efficient (H)CCH correlations detected on side chain protons. Our approach is demonstrated on the HET-s prion domain in its functional amyloid fibrillar form, fully protonated, with a sample amount of less than 500 g using a MAS frequency of 70kHz. The majority of aliphatic and aromatic side chain protons (70%) are observable, in addition to Halpha resonances, in a single experiment providing a complementary approach to the established proton-detected amide-based multidimensional solid-state NMR experiments for the study and resonance assignment of biosolid samples, in particular for aromatic side chain resonances.
dc.language.isoENen_US
dc.subject.enAmyloid fibrils
dc.subject.enSolid-state NMR
dc.subject.enProton detection
dc.subject.enVery fast MAS
dc.subject.enProtein NMR
dc.title.enDetection of side-chain proton resonances of fully protonated biosolids in nano-litre volumes by magic angle spinning solid-state NMR
dc.title.alternativeJ Biomol NMRen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1007/s10858-018-0168-3en_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalJournal of biomolecular NMRen_US
bordeaux.page177-185en_US
bordeaux.volume70en_US
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-02536705
hal.version1
hal.date.transferred2020-04-08T10:11:53Z
hal.exporttrue
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