A critical assessment of transmethylation procedures for n-3 long-chain polyunsaturated fatty acid quantification of lipid classes
dc.rights.license | open | en_US |
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hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
hal.structure.identifier | ITERG | |
dc.contributor.author | SEHL, Anthony | |
hal.structure.identifier | ITERG | |
dc.contributor.author | COUEDELO, Leslie | |
hal.structure.identifier | ITERG | |
dc.contributor.author | FONSECA, Laurence | |
hal.structure.identifier | ITERG | |
dc.contributor.author | VAYSSE, Carole | |
hal.structure.identifier | Chimie et Biologie des Membranes et des Nanoobjets [CBMN] | |
dc.contributor.author | CANSELL, Maud | |
dc.date.accessioned | 2020-04-08T09:12:23Z | |
dc.date.available | 2020-04-08T09:12:23Z | |
dc.date.issued | 2018 | |
dc.identifier.issn | 0308-8146 | en_US |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/4161 | |
dc.description.abstractEn | Lipid transmethylation methods described in the literature are not always evaluated with care so to insure that the methods are effective, especially on food matrix or biological samples containing polyunsaturated fatty acid (PUFA). The aim of the present study was to select a method suitable for all lipid species rich in long chain n-3 PUFA. Three published methods were adapted and applied on individual lipid classes. Lipid (trans)methylation efficiency was characterized in terms of reaction yield and gas chromatography (GC) analysis. The acid-catalyzed method was unable to convert triglycerides and sterol esters, while the method using an incubation at a moderate temperature was ineffective on phospholipids and sterol esters. On the whole only the method using sodium methoxide and sulfuric acid was effective on lipid classes taken individually or in a complex medium. This study highlighted the use of an appropriate (trans)methylation method for insuring an accurate fatty acid composition. | |
dc.language.iso | EN | en_US |
dc.subject.en | Methylation | |
dc.subject.en | Fatty acid methyl esters | |
dc.subject.en | Polyunsaturated fatty acids | |
dc.subject.en | Gas chromatography | |
dc.subject.en | High-performance thin layer chromatography | |
dc.title.en | A critical assessment of transmethylation procedures for n-3 long-chain polyunsaturated fatty acid quantification of lipid classes | |
dc.type | Article de revue | en_US |
dc.identifier.doi | 10.1016/j.foodchem.2018.01.060 | en_US |
dc.subject.hal | Chimie/Matériaux | en_US |
bordeaux.journal | Food Chemistry | en_US |
bordeaux.page | 1-8 | en_US |
bordeaux.volume | 251 | en_US |
bordeaux.hal.laboratories | Institut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248 | |
bordeaux.institution | Bordeaux INP | en_US |
bordeaux.institution | Université de Bordeaux | en_US |
bordeaux.peerReviewed | oui | en_US |
bordeaux.inpress | non | en_US |
hal.identifier | hal-02536529 | |
hal.version | 1 | |
hal.date.transferred | 2020-04-08T09:12:28Z | |
hal.export | true | |
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