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Comparative study of strong cation exchangers: Structure-related chromatographic performances

dc.rights.licenseopenen_US
dc.contributor.authorJANAKIRAMAN, Vignesh Narasimhan
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorSOLE, Marion
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorMARIA, Sophie
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorPEZZINI, Jerome
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorCABANNE, Charlotte
hal.structure.identifierChimie et Biologie des Membranes et des Nanoobjets [CBMN]
dc.contributor.authorSANTARELLI, Xavier
dc.date.accessioned2020-04-07T12:58:36Z
dc.date.available2020-04-07T12:58:36Z
dc.date.issued2018
dc.identifier.issn1570-0232en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/4148
dc.description.abstractEnChromatographic performances are highly influenced by operational parameters. New ion exchangers have tailored matrices providing low backpressure, thereby allowing high flow velocity. By systematic frontal analysis and selectivity determination at different flow rates, we independently evaluated cation exchangers to facilitate media selection and investigated the relationship between surface modification and chromatographic performances. Structure-extended resins showed higher binding capacities compared to resins with conventional ligands directly attached to the matrix. Moreover, they maintained high capacities even with high flow velocities. Ligand accessibility was therefore largely enhanced, allowing proteins to interact and bind under harsh conditions with minimal residence/contact time. High throughput resins can be used for purification of high volume and high concentration feedstock in limited time. This results in higher productivity, and could contribute to cost reduction. In this work, we evaluated the dynamic binding capacities of various new ion exchange resins at different binding conductivities for different residence times, and observed that.
dc.language.isoENen_US
dc.subject.enCation exchange
dc.subject.enResolution
dc.subject.enDynamic binding capacity
dc.subject.enFlow rate
dc.title.enComparative study of strong cation exchangers: Structure-related chromatographic performances
dc.typeArticle de revueen_US
dc.identifier.doi10.1016/j.jchromb.2018.02.014en_US
dc.subject.halChimie/Matériauxen_US
bordeaux.journalJournal of chromatography. B, Analytical technologies in the biomedical and life sciencesen_US
bordeaux.page1-10en_US
bordeaux.volume1080en_US
bordeaux.hal.laboratoriesInstitut de Chimie & de Biologie des Membranes & des Nano-objets (CBMN) - UMR 5248
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-02535242
hal.version1
hal.date.transferred2020-04-07T12:58:40Z
hal.exporttrue
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