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Low source–sink ratio reduces reserve starch in grapevine woody canes and modulates sugar transport and metabolism at transcriptional and enzyme activity levels

dc.rights.licenseopenen_US
dc.contributor.authorSILVA, Angélica
dc.contributor.authorNORONHA, Henrique
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorDAI, Zhanwu
IDREF: 22822473X
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorDELROT, Serge
IDREF: 058711503
dc.contributor.authorGERÓS, Hernâni
dc.date.accessioned2020-04-02T13:45:40Z
dc.date.available2020-04-02T13:45:40Z
dc.date.issued2017
dc.identifier.issn0032-0935en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/4076
dc.description.abstractEnMain conclusionSevere leaf removal decreases storage starch and sucrose in grapevine cv. Cabernet Sauvignon fruiting cuttings and modulates the activity of key enzymes and the expression of sugar transporter genes.[br/] Leaf removal is an agricultural practice that has been shown to modify vineyard efficiency and grape and wine composition. In this study, we took advantage of the ability to precisely control the number of leaves to fruits in Cabernet Sauvignon fruiting cuttings to study the effect of source–sink ratios (2 (2L), 6 (6L) and 12 (12) leaves per cluster) on starch metabolism and accumulation. Starch concentration was significantly higher in canes from 6L (42.13 ± 1.44 mg g DW−1) and 12L (43.50 ± 2.85 mg g DW−1) than in 2L (22.72 ± 3.10 mg g DW−1) plants. Moreover, carbon limitation promoted a transcriptional adjustment of genes involved in starch metabolism in grapevine woody tissues, including a decrease in the expression of the plastidic glucose-6-phosphate translocator, VvGPT1. Contrarily, the transcript levels of the gene coding the catalytic subunit VvAGPB1 of the VvAGPase complex were higher in canes from 2L plants than in 6L and 12L, which positively correlated with the biochemical activity of this enzyme. Sucrose concentration increased in canes from 2L to 6L and 12L plants, and the amount of total phenolics followed the same trend. Expression studies showed that VvSusy transcripts decreased in canes from 2L to 6L and 12L plants, which correlated with the biochemical activity of insoluble invertase, while the expression of the sugar transporters VvSUC11 and VvSUC12, together with VvSPS1, which codes an enzyme involved in sucrose synthesis, increased. Thus, sucrose seems to control starch accumulation through the adjustment of the cane sink strength.
dc.language.isoENen_US
dc.subjectVitis Vinifera
dc.subjectSaccharose
dc.subjectExpression des gènes
dc.subjectTraitement des feuilles
dc.subject.enLeaf–Cluster Ratio
dc.subject.enLeaf Removal
dc.subject.enStarch
dc.subject.enViticulture
dc.subject.enVitis Vinifera
dc.title.enLow source–sink ratio reduces reserve starch in grapevine woody canes and modulates sugar transport and metabolism at transcriptional and enzyme activity levels
dc.typeArticle de revueen_US
dc.identifier.doi10.1007/s00425-017-2708-6
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
bordeaux.journalPlantaen_US
bordeaux.page525-535en_US
bordeaux.volume246en_US
bordeaux.hal.laboratoriesEcophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287en_US
bordeaux.issue3en_US
bordeaux.institutionBordeaux Sciences Agroen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-03170560
hal.version1
hal.date.transferred2021-03-16T10:54:17Z
hal.exporttrue
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