Root transcriptomic responses of grafted grapevines to heterogeneous nitrogen availability depend on rootstock genotype
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Article de revue
Este ítem está publicado en
Journal of Experimental Botany. 2017, vol. 68, n° 15, p. 4339 - 4355
Resumen en inglés
In many fruit species, including grapevine, grafting is used to improve scion productivity and quality and to adapt the plant to environmental conditions. However, the mechanisms underlying the rootstock control of scion ...Leer más >
In many fruit species, including grapevine, grafting is used to improve scion productivity and quality and to adapt the plant to environmental conditions. However, the mechanisms underlying the rootstock control of scion development are still poorly understood. The ability of rootstocks to regulate nitrogen uptake and assimilation may contribute to this control. A split-root system was used to grow heterografted grapevines and to investigate the molecular responses to changes in nitrate availability of two rootstocks known to affect scion growth differently. Transcriptome profiling by RNA sequencing was performed on root samples collected 3 and 24 h after nitrogen supply. The results demonstrated a common response involving nitrogen-related genes, as well as a more pronounced transcriptomic reprogramming in the genotype conferring the lower scion growth. A weighted gene co-expression network analysis allowed the identification of co-regulated gene modules, suggesting a role for nitrate transporter 2 family genes and some transcription factors as main actors controlling this genotype-dependent response to heterogeneous nitrogen supply. The relationship between nitrate, ethylene, and strigolactone hormonal pathways was found to differ between the two genotypes. These findings indicated that the genotypes responded differently to heterogeneous nitrogen availability, and this may contribute to their contrasting effect on scion growth.< Leer menos
Palabras clave en inglés
Grafted Plants
Grapevine
Nitrate
Rna-Seq
Rootstocks
Split-Root System
Transcriptome
Arabidopsis-Thaliana Roots
Nitrate Transporter Nrt2.1
Gene-Expression Data
Rna-Seq
Signaling Pathway
Plant Development
Abscisic-Acid
Shoot
Scion
Cytokinin
Centros de investigación