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Roles of dicer-like proteins 2 and 4 in intra- and intercellular antiviral silencing

dc.rights.licenseopenen_US
dc.contributor.authorQIN, Cheng
dc.contributor.authorLI, Bin
dc.contributor.authorFAN, Yaya
dc.contributor.authorZHANG, Xian
dc.contributor.authorYU, Zhiming
dc.contributor.authorRYABOV, Eugene
dc.contributor.authorZHAO, Mei
dc.contributor.authorWANG, Hui
dc.contributor.authorSHI, Nongnong
dc.contributor.authorZHANG, Pengcheng
dc.contributor.authorJACKSON, Stephen
dc.contributor.authorTÖR, Mahmut
dc.contributor.authorCHENG, Qi
dc.contributor.authorLIU, Yule
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorGALLUSCI, Philippe
dc.contributor.authorHONG, Yiguo
dc.date.accessioned2020-04-01T12:38:46Z
dc.date.available2020-04-01T12:38:46Z
dc.date.issued2017
dc.identifier.issn0032-0889en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/4050
dc.description.abstractEnRNA silencing is an innate antiviral mechanism conserved in organisms across kingdoms. Such a cellular defense involves DICER or DICER-LIKEs (DCLs) that process plant virus RNAs into viral small interfering RNAs (vsiRNAs). Plants encode four DCLs that play diverse roles in cell-autonomous intracellular virus-induced RNA silencing (known as VIGS) against viral invasion. VIGS can spread between cells. However, the genetic basis and involvement of vsiRNAs in non-cell-autonomous intercellular VIGS remains poorly understood. Using GFP as a reporter gene together with a suite of DCL RNAi transgenic lines, here we show that despite the well-established activities of DCLs in intracellular VIGS and vsiRNA biogenesis, DCL4 acts to inhibit intercellular VIGS whereas DCL2 is required (likely along with DCL2-processed/dependent vsiRNAs and their precursor RNAs) for efficient intercellular VIGS trafficking from epidermal to adjacent cells. DCL4 imposed an epistatic effect on DCL2 to impede cell-to-cell spread of VIGS. Our results reveal previously unknown functions for DCL2 and DCL4 that may form a dual defensive frontline for intra- and intercellular silencing to double-protect cells from virus infection in Nicotiana benthamiana.
dc.language.isoENen_US
dc.subjectBiogénèse
dc.subjectInfection virale
dc.subjectArn
dc.subjectNicotiana Benthamiana
dc.title.enRoles of dicer-like proteins 2 and 4 in intra- and intercellular antiviral silencing
dc.title.alternativePlant physiol.en_US
dc.typeArticle de revueen_US
dc.identifier.doi10.1104/pp.17.00475
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
dc.identifier.pubmed28455401en_US
bordeaux.journalPlant Physiologyen_US
bordeaux.page1067-1081en_US
bordeaux.volume174en_US
bordeaux.hal.laboratoriesEcophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287en_US
bordeaux.issue2en_US
bordeaux.institutionBordeaux Sciences Agroen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-03170486
hal.version1
hal.date.transferred2021-03-16T10:17:23Z
hal.exporttrue
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