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Temporal kinetics of the transcriptional response to carbon depletion and sucrose readdition in Arabidopsis seedlings

dc.rights.licenseopenen_US
hal.structure.identifierEcophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]
dc.contributor.authorCOOKSON, Sarah
IDREF: 22161009X
dc.contributor.authorKLIE, Sebastian
dc.contributor.authorMORCUENDE, Rosa
dc.contributor.authorUSADEL, Björn
dc.contributor.authorLUNN, John Edward
dc.contributor.authorSTITT, Mark
dc.date.accessioned2020-03-29T13:01:36Z
dc.date.available2020-03-29T13:01:36Z
dc.date.issued2016
dc.identifier.issn0140-7791en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/4012
dc.description.abstractEnTo investigate whether the transcriptional response to carbon (C) depletion and sucrose resupply depends on the duration and severity of the C depletion, Arabidopsis seedlings were grown in liquid culture and harvested 3, 6, 12, 24, 48 and 72 h after removing sucrose from the medium and 30 min after resupplying sucrose at each time. Expression profiling revealed early transcriptional inhibition of cell wall synthesis and remodelling of signalling, followed by induction of C recycling and photosynthesis and general inhibition of growth. The temporal sequence differed from the published response to progressive exhaustion of C during a night and extended night in vegetatively growing plants. The response to sucrose readdition was conserved across the C-depletion time course. Intriguingly, the vast majority of rapidly responding transcripts decreased rather than increased. The majority of transcripts that respond rapidly to sucrose and many transcripts that respond during C depletion also decrease after treating seedlings with the transcriptional inhibitor cordycepin A. Comparison with published responses to overexpression of otsA, AKIN10 and bZIP11 revealed that many genes that respond to C depletion, and especially sucrose resupply, respond to one or more of these C-signalling components. Thus, multiple factors contribute to C responsiveness, including many signalling components, transcriptional regulation and transcript turnover.
dc.language.isoENen_US
dc.subjectAnalyse transcriptionnelle
dc.subjectCarbone
dc.subjectArabidopsis
dc.subjectParoi cellulaire
dc.subjectSaccharose
dc.subjectInhibition
dc.subject.enCarbohydrate Starvation
dc.subject.enSucrose Readdition
dc.subject.enTranscriptional Responses
dc.subject.enArabidopsis Seedlings
dc.title.enTemporal kinetics of the transcriptional response to carbon depletion and sucrose readdition in Arabidopsis seedlings
dc.typeArticle de revueen_US
dc.identifier.doi10.1111/pce.12642en_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
bordeaux.journalPlant, Cell and Environmenten_US
bordeaux.page768-786en_US
bordeaux.volume39en_US
bordeaux.hal.laboratoriesEcophysiologie et Génomique Fonctionnelle de la Vigne (EGFV) - UMR 1287en_US
bordeaux.issue4en_US
bordeaux.institutionBordeaux Sciences Agroen_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
hal.identifierhal-02523674
hal.version1
hal.date.transferred2020-03-29T13:01:40Z
hal.exporttrue
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