DAI, Zhanwu; PLESSIS, Anne; VINCENT, Jonathan; DUCHATEAU, Nathalie; BESSON, Alicia; DARDEVET, Mireille; PRODHOMME, Duyên; GIBON, Yves; GODINAUD, Ghislaine; PAILLOUX, Marie; GRAND RAVEL, Catherine; MARTRES, Pierre

doi:10.1111/tpj.12881

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DAI, Zhanwu

Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]

PLESSIS, Anne

VINCENT, Jonathan

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Article de revue

Ce document a été publié dans

Plant Journal. 2015, vol. 83, n° 2, p. 326-343

Résumé en anglais

Wheat (Triticum aestivum L.) grain storage proteins (GSPs) are major determinants of flour end-use value. Biological and molecular mechanisms underlying the developmental and nutritional determination of GSP accumulation in cereals are as yet poorly understood. Here we timed the accumulation of GSPs during wheat grain maturation relative to changes in metabolite and transcript pools in different conditions of nitrogen (N) and sulfur (S) availability. We found that the N/S supply ratio modulated the duration of accumulation of S-rich GSPs and the rate of accumulation of S-poor GSPs. These changes are likely to be the result of distinct relationships between N and S allocation, depending on the S content of the GSP. Most developmental and nutritional modifications in GSP synthesis correlated with the abundance of structural gene transcripts. Changes in the expression of transport and metabolism genes altered the concentrations of several free amino acids under variable conditions of N and S supply, and these amino acids seem to be essential in determining GSP expression. The comprehensive data set generated and analyzed here provides insights that will be useful in adapting fertilizer use to variable N and S supply, or for breeding new cultivars with balanced and robust GSP composition.< Réduire

Mots clés

Triticum Aestivum

Farine

Protéine

Métabolite

Pcr

Céréale stockée

Teneur en soufre

Analyse intégrative

Disponibilité en azote

Expression des gènes

Mots clés en anglais

Triticum Aestivum

Integrative Analysis

Nitrogen

Seed Storage Protein

Sulfur Deficiency