LIU, Liwang; PLAWINSKI, Laurent; DURRIEU, Marie‐Christine; AUDOIN, Bertrand

doi:10.1002/jbio.201900045

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LIU, Liwang

PLAWINSKI, Laurent

DURRIEU, Marie‐Christine

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Ce document a été publié dans

Journal of Biophotonics. 2019, vol. 12, n° 8, p. 11 p.

Résumé en anglais

Advances in microscopy with new visualization possibilities often bring dramatic progress to our understanding of the intriguing cellular machinery. Picosecond optoacoustic micro‐spectroscopy is an optical technique based on ultrafast pump‐probe generation and detection of hypersound on time durations of picoseconds and length scales of nanometers. It is experiencing a renaissance as a versatile imaging tool for cell biology research after a plethora of applications in solid‐state physics. In this emerging context, this work reports on a dual‐probe architecture to carry out real‐time parallel detection of the hypersound propagation inside a cell that is cultured on a metallic substrate, and of the hypersound reflection at the metal/cell adhesion interface. Using this optoacoustic modality, several biophysical properties of the cell can be measured in a noncontact and label‐free manner. Its abilities are demonstrated with the multiple imaging of a mitotic macrophage‐like cell in a single run experiment.< Réduire

Mots clés en anglais

cell adhesion

cell mechanics

mitosis and division

picosecond optoacoustics

single‐cell imaging

Project ANR

Echographie opto-acoustique de cellules individuelles ex vivo - ANR-17-CE11-0020

Unités de recherche

CBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248