Coiled-coil oligomerization controls localization of the plasma membrane REMORINs.
Language
EN
Article de revue
This item was published in
Journal of Structural Biology. 2019, vol. 206, n° 1, p. 12-19
English Abstract
REMORINs are nanodomain-organized proteins located in the plasma membrane and involved in cellular re-sponses in plants. The dynamic assembly of the membrane nanodomains represents an essential tool of theversatile membrane ...Read more >
REMORINs are nanodomain-organized proteins located in the plasma membrane and involved in cellular re-sponses in plants. The dynamic assembly of the membrane nanodomains represents an essential tool of theversatile membrane barriers to control and modulate cellular functions. Nevertheless, the assembly mechanismsand protein organization strategies of nanodomains are poorly understood and many structural aspects aredifficult to visualize. Using an ensemble of biophysical approaches, including solid-state nuclear magnetic re-sonance, cryo-electron microscopy andin vivoconfocal imaging, we providefirst insights on the role and thestructural mechanisms of REMORIN trimerization. Our results suggest that the formation of REMORIN coiled-coil trimers is essential for membrane recruitment and promotes REMORIN assemblyin vitrointo longfilamentsby trimer-trimer interactions that might participate in nanoclustering into membrane domainsin vivo.Read less <
English Keywords
REMORIN
Nanodomain
Membrane protein
Solid-state NMR
Protein filament