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FBXO32 links ubiquitination to epigenetic reprograming of melanoma cells

dc.rights.licenseopenen_US
dc.contributor.authorHABEL, Nadia
dc.contributor.authorEL-HACHEM, Najla
dc.contributor.authorSOYSOUVANH, Frederic
dc.contributor.authorHADHIRI-BZIOUECHE, Hanene
dc.contributor.authorGIULIANO, Serena
dc.contributor.authorNGUYEN, Sophie
dc.contributor.authorHORAK, Pavel
dc.contributor.authorGAY, Anne-Sophie
dc.contributor.authorDEBAYLE, Delphine
dc.contributor.authorNOTTET, Nicolas
dc.contributor.authorBERANGER, Guillaume
hal.structure.identifierBordeaux population health [BPH]
dc.contributor.authorBRESSAC-DE PAILLERETS, Brigitte
dc.contributor.authorBERTOLOTTO, Corine
dc.contributor.authorBALLOTTI, Robert
dc.date.accessioned2021-05-06T12:07:23Z
dc.date.available2021-05-06T12:07:23Z
dc.date.issued2020-12
dc.identifier.issn1350-9047en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/27169
dc.description.abstractEnUbiquitination by serving as a major degradation signal of proteins, but also by controlling protein functioning and localization, plays critical roles in most key cellular processes. Here, we show that MITF, the master transcription factor in melanocytes, controls ubiquitination in melanoma cells. We identified FBXO32, a component of the SCF E3 ligase complex as a new MITF target gene. FBXO32 favors melanoma cell migration, proliferation, and tumor development in vivo. Transcriptomic analysis shows that FBXO32 knockdown induces a global change in melanoma gene expression profile. These include the inhibition of CDK6 in agreement with an inhibition of cell proliferation and invasion upon FBXO32 silencing. Furthermore, proteomic analysis identifies SMARC4, a component of the chromatin remodeling complexes BAF/PBAF, as a FBXO32 partner. FBXO32 and SMARCA4 co-localize at loci regulated by FBXO32, such as CDK6 suggesting that FBXO32 controls transcription through the regulation of chromatin remodeling complex activity. FBXO32 and SMARCA4 are the components of a molecular cascade, linking MITF to epigenetics, in melanoma cells.
dc.language.isoENen_US
dc.rightsAttribution 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/*
dc.subject.enEpigenetics
dc.subject.enOncogenes
dc.title.enFBXO32 links ubiquitination to epigenetic reprograming of melanoma cells
dc.typeArticle de revueen_US
dc.identifier.doi10.1038/s41418-020-00710-xen_US
dc.subject.halSciences du Vivant [q-bio]/Canceren_US
dc.identifier.pubmed33462405en_US
bordeaux.journalCell Death and Differentiationen_US
bordeaux.pageOnline ahead of printen_US
bordeaux.hal.laboratoriesBordeaux Population Health Research Center (BPH) - U1219en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionINSERMen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.identifier.funderIDLigue Contre le Canceren_US
bordeaux.import.sourcehal
hal.identifierinserm-03138835
hal.version1
hal.exportfalse
workflow.import.sourcehal
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Cell%20Death%20and%20Differentiation&rft.date=2020-12&rft.spage=Online%20ahead%20of%20print&rft.epage=Online%20ahead%20of%20print&rft.eissn=1350-9047&rft.issn=1350-9047&rft.au=HABEL,%20Nadia&EL-HACHEM,%20Najla&SOYSOUVANH,%20Frederic&HADHIRI-BZIOUECHE,%20Hanene&GIULIANO,%20Serena&rft.genre=article


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