LAURA, Chaillot; MARIE-LISE, Blondot; PATRICIA, Recordon-Pinson; ISABELLE, Pellegrin; ANDREA, Boizard-Moracchini; MYROSLAVA, Sliusar; TEO, Leboucq; NADEGE, Pujol; MARIE-LINE, Andreola; FABRICE, Bonnet; GAELLE, Recher; LAETITIA, Andrique; PIERRE, Nassoy; THOMAS, Mathivet; ANDREAS, Bikfalvi

doi:10.1038/s41598-025-08329-z

LAURA, Chaillot
BoRdeaux Institute in onCology [Inserm U1312 - BRIC]

MARIE-LISE, Blondot
Microbiologie Fondamentale et Pathogénicité [MFP]

PATRICIA, Recordon-Pinson
Microbiologie Fondamentale et Pathogénicité [MFP]
TBM-Core [Bordeaux] [UMS3427 - INSERM US005]

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Article de revue

Este ítem está publicado en

Scientific Reports. 2025-07-01, vol. 15, n° 1, p. 21129

Resumen en inglés

The blood vessel network is heavily impacted by SARS-CoV-2 infection. How SARS-CoV-2 contributes to vascular inflammation and whether epithelio-endothelial cross-talk is involved remain unclear. We investigated in detail the interaction between SARS-CoV-2 and the vasculature using 2D and 3D vesseloid in vitro models. We first assessed whether SARS-CoV-2 is able to directly infect endothelial cells. In the absence of ACE2 in endothelial cells, no productive infection was detected. Low uptake of viral particles by ACE2-overexpressing endothelial cells was observed without efficient viral production. Thus, the indirect effect of SARS-CoV-2 infection may involve epithelio-endothelial cell cross-talk. After infection of the epithelial cells, a significant inflammatory response was detected in the endothelial cells. CXCL10 was the most highly expressed proinflammatory cytokine involved in this intercellular communication, and its function was subsequently explored. Finally, the clinical relevance of our findings was confirmed in two patient cohorts.< Leer menos

Palabras clave en inglés

Humans

COVID-19

Chemokine CXCL10

SARS-CoV-2

Endothelial Cells

Cell Communication

Angiotensin-Converting Enzyme 2

Epithelial Cells

Human Umbilical Vein Endothelial Cells

Endothelium

Vascular

URI

https://oskar-bordeaux.fr/handle/20.500.12278/207344

DOI

http://dx.doi.org/10.1038/s41598-025-08329-z

Centros de investigación

MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234

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CXCL10-dependent epithelial-vascular cross-talk for endothelial activation following SARS-CoV-2 infection.

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Resumen en inglés

Palabras clave en inglés

URI

DOI

Centros de investigación