CAMBOUE, Marilou; JANOUEIX, Anne; TANDONNET, Jean-Pascal; SPILMONT, Anne-Sophie; MOISY, Cédric; MATHIEU, Guillaume; CORDELIERES, Fabrice; TEILLON, Jérémie; GONZAGA SANTESTEBAN, Luis; OLLAT, Nathalie; COOKSON, Sarah Jane

doi:10.1111/pce.14883

CAMBOUE, Marilou
Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]

JANOUEIX, Anne

Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]

TANDONNET, Jean-Pascal
Ecophysiologie et Génomique Fonctionnelle de la Vigne [UMR EGFV]

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Article de revue

Ce document a été publié dans

Plant, Cell and Environment. 2024-07, vol. 47, n° 7, p. 2349-2359

Résumé en anglais

Plants are able to naturally graft or inosculate their trunks, branches and roots together, this mechanism is used by humans to graft together different genotypes for a range of purposes. Grafts are considered successful if functional vascular connections between the two genotypes occur. Various techniques can evaluate xylem connections across the graft interface. However, these methods are generally unable to assess the heterogeneity and three-dimensional (3D) structure of xylem vessel connections. Here we present the use of X-ray micro-computed tomography to characterize the 3D morphology of grafts of grapevine. We show that xylem vessels form between the two plants of natural root and human-made stem grafts. The main novelty of this methodology is that we were able to visualize the 3D network of functional xylem vessels connecting the scion and rootstock in human-made stem grafts thanks to the addition of a contrast agent to the roots and improved image analysis pipelines. In addition, we reveal the presence of extensive diagonal xylem connections between the main axial xylem vessels in 2-year old grapevine stems. In conclusion, we present a method that has the potential to provide new insights into the structure and function of xylem vessels in large tissue samples. © 2024 The Authors. Plant, Cell & Environment published by John Wiley & Sons Ltd.< Réduire

Mots clés en anglais

Grafting

Grapevine

Scion

Stock

3D Imaging

Micro-CT

URI

https://oskar-bordeaux.fr/handle/20.500.12278/206516

DOI

http://dx.doi.org/10.1111/pce.14883

Project ANR

Développment d'une infrastructure française distribuée coordonnée - ANR-10-INBS-0004
Mediterranean Center for Environment and Biodiversity - ANR-10-LABX-0004
Digital and Hardware Solutions and Modeling for the Environement and Life Sciences - ANR-10-LABX-0020

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Phenotyping xylem connections in grafted plants using X-ray micro-computed tomography

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Résumé en anglais

Mots clés en anglais

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Licence d’utilisation du document

Phenotyping xylem connections in grafted plants using X-ray micro-computed tomography

Langue

Ce document a été publié dans

Résumé en anglais

Mots clés en anglais

URI

DOI

Project ANR

Unités de recherche