This study focuses on countering Fusarium graminearum, a harmful fungal pathogen impacting cereal crops and human health through mycotoxin production. These mycotoxins, categorized as type B trichothecenes, pose significant health risks. Research explores natural alternatives to synthetic fungicides, particularly investigating phenolics in grapevine byproducts. Thirteen eco-extracts from five French grape varieties (Merlot, Cabernet Sauvignon, Sauvignon blanc, Tannat, and Artaban) exhibited substantial antifungal properties, with ten extracts displaying remarkable effects. Extracts from grapevine stems and roots notably reduced fungal growth by over 91% after five days. Through UHPLC-HRMS/MS analysis and metabolomics, the study identified potent antifungal compounds such as ampelopsin A and cyphostemmin B, among other oligomeric stilbenes. Interestingly, this approach showed that flavan-3-ols have been identified as markers for extracts that induce fungal growth. Root extracts from rootstocks, rich in oligostilbenes, demonstrated the highest antifungal activity. This research underscores grapevine byproducts’ potential both as a sustainable approach to control F. graminearum and mycotoxin contamination in cereal crops and the presence of different metabolites from the cultivars of grapevine, suggesting different activities. © 2024 American Chemical Society.< Réduire

Mots clés en anglais

Vitis vinifera

Byproducts

Stilbenes

MS/MS-based untargeted metabolomics

Fusarium graminearum

Antifungal activity

URI

https://oskar-bordeaux.fr/handle/20.500.12278/206238

DOI

http://dx.doi.org/10.1021/acs.jafc.3c09531

Project ANR

Les stilbénoïdes: produits naturels prometteurs, respectueux de l'environnement, destinés à réduire la contamination du blé et du raisin par les champignons toxinogènes et les mycotoxines associées - ANR-19-CE21-0007
Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010
Centre français de phénomique végétale - ANR-11-INBS-0012

Unités de recherche

Oenologie - UMR 1366

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Targeting of Antifungal Metabolites from Grapevine Byproducts by UPLC–HRMS/MS Approaches Using Bioactivity-Based Molecular Networking

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