KOSOVARI, Melissa; BUFFETEAU, Thierry; THOMAS, Laurent; GUAY-BEGIN, Andree-Anne; VELLUTINI, Luc; MCGETTRICK, James; LAROCHE, Gaetan; DURRIEU, Marie-Christine

doi:10.1021/acsami.4c03727

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KOSOVARI, Melissa
Chimie et Biologie des Membranes et des Nanoobjets [CBMN]

BUFFETEAU, Thierry

THOMAS, Laurent

Langue

Article de revue

Ce document a été publié dans

ACS Applied Materials & Interfaces. 2024-06-04, vol. 16, n° 23, p. 29770-29782

Résumé en anglais

Biomaterial surface engineering and the integration of cell-adhesive ligands are crucial in biological research and biotechnological applications. The interplay between cells and their microenvironment, influenced by chemical and physical cues, impacts cellular behavior. Surface modification of biomaterials profoundly affects cellular responses, especially at the cell–surface interface. This work focuses on enhancing cellular activities through material manipulation, emphasizing silanization for further functionalization with bioactive molecules such as RGD peptides to improve cell adhesion. The grafting of three distinct silanes onto silicon wafers using both spin coating and immersion methods was investigated. This study sheds light on the effects of different alkyl chain lengths and protecting groups on cellular behavior, providing valuable insights into optimizing silane-based self-assembled monolayers (SAMs) before peptide or protein grafting for the first time. Specifically, it challenges the common use of APTES molecules in this context. These findings advance our understanding of surface modification strategies, paving the way for tailoring biomaterial surfaces to modulate the cellular behavior for diverse biotechnological applications.< Réduire

Mots clés en anglais

Biomaterial surface engineering

Biomaterial surface engineering Silanization Integrin-based ligands Surface functionalization Cell adhesion

Cell adhesion

Integrin-based ligands

Silanization

Surface functionalization

URI

https://oskar-bordeaux.fr/handle/20.500.12278/205976

DOI

http://dx.doi.org/10.1021/acsami.4c03727

Project ANR

Conception de surfaces bioinspirées avec des propriétés mécaniques et de bioactivité contrôlées pour la synthèse de plateforme in vitro de culture cellulaire - ANR-21-CE06-0031

Unités de recherche

CBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248