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The intracellular drug delivery and anti tumor activity of doxorubicin loaded poly(gamma-benzyl L-glutamate)-b-hyaluronan polymersomes

dc.rights.licenseopen
hal.structure.identifierPharm Dept, Fac Technol & Eng, Univ Baroda
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
dc.contributor.authorUPADHYAY, Kamal Kumar
hal.structure.identifierDiv Radiat Biosci [DRDO, INMAS]
dc.contributor.authorBHATT, Anant N.
hal.structure.identifierDiv Cyclotron & Radiopharmaceut Sci [DRDO, INMAS]
dc.contributor.authorMISHRA, Anil K.
hal.structure.identifierDiv Radiat Biosci [DRDO, INMAS]
dc.contributor.authorDWARAKANATH, Bilikere
hal.structure.identifierNatl Inst Pharmaceut Educ & Res
dc.contributor.authorJAIN, Sanyog
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorSCHATZ, Christophe
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorLE MEINS, Jean-Francois
hal.structure.identifierDiv Radiat Biosci [DRDO, INMAS]
dc.contributor.authorFAROOQUE, Abdullah
hal.structure.identifierNatl Inst Pharmaceut Educ & Res
dc.contributor.authorCHANDRAIAH, Godugu
hal.structure.identifierNatl Inst Pharmaceut Educ & Res
dc.contributor.authorJAIN, Amit K.
hal.structure.identifierPharm Dept, Fac Technol & Eng, Univ Baroda
dc.contributor.authorMISRA, Ambikanandan
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorLECOMMANDOUX, Sebastien
dc.date.accessioned2020
dc.date.available2020
dc.date.created2010
dc.date.issued2010
dc.identifier.issn0142-9612
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/20556
dc.description.abstractEnWe have investigated the intracellular delivery of doxorubicin (DOX) loaded poly(gamma-benzyl L-glutamate)block-hyaluronan (PBLG-b-HYA) based polymersomes (PolyDOX) in high (MCF-7) and low (U87) CD44 expressing cancer cell models. DOX was successfully loaded into polymersomes using nanoprecipitation method and in vitro drug release pattern were achieved at pH 5.5 and 7.4 up to 10 days. Block copolymer vesicles without loaded DOX were non cytotoxic in both cells at concentration 150-650 mu g/mL. Flow cytometry data suggested successful uptake of PolyDOX in cells and high accumulation was found in MCF-7 than U87 cells. Microscopy imagings revealed that in MCF-7 cells PolyDOX was more in cytoplasm and free DOX in nuclei, whereas in U87 cells free DOX was also found in the cytoplasm. Cytotoxicity of the drug was concentration and exposure time dependent. In addition, PolyDOX significantly enhanced reactive oxygen species (ROS) level in both cells. PolyDOX also suppressed growth of breast tumor on female Sprague-Dawley (SD) rats as compared to phosphate buffer saline pH 7.4 (PBS) control group. In addition reduced level of serum enzymes (LDH and CPK) by PolyDOX formulation indicated less cardiotoxicity of DOX after loading in polymersomes. Results suggest that intracellular delivery of PolyDOX was depended on the CD44 expression level in cells due to, presence of hyaluronic acid on the surface of polymersomes, and could be used as a self-targeting drug delivery cargo in over-expressed CD44 glycoprotein cells of breast cancer.
dc.language.isoen
dc.publisherElsevier
dc.subject.enPolymersomes
dc.subject.enHyaluronan
dc.subject.enDoxorubicin
dc.subject.enCell uptake
dc.subject.enCytotoxicity
dc.subject.enSelf-assembly
dc.title.enThe intracellular drug delivery and anti tumor activity of doxorubicin loaded poly(gamma-benzyl L-glutamate)-b-hyaluronan polymersomes
dc.typeArticle de revue
dc.identifier.doi10.1016/j.biomaterials.2009.12.043
dc.subject.halChimie/Polymères
bordeaux.journalBiomaterials
bordeaux.page2882-2892
bordeaux.volume31
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue10
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-00652923
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-00652923v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Biomaterials&rft.date=2010&rft.volume=31&rft.issue=10&rft.spage=2882-2892&rft.epage=2882-2892&rft.eissn=0142-9612&rft.issn=0142-9612&rft.au=UPADHYAY,%20Kamal%20Kumar&BHATT,%20Anant%20N.&MISHRA,%20Anil%20K.&DWARAKANATH,%20Bilikere&JAIN,%20Sanyog&rft.genre=article


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