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Cellular Uptake and Cytotoxic Effect of Epidermal Growth Factor Receptor Targeted and Plitidepsin Loaded Co-Polymeric Polymersomes on Colorectal Cancer Cell Lines

dc.rights.licenseopen
hal.structure.identifierGAIKER Technological Centre
dc.contributor.authorGONI-DE-CERIO, Felipe
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorTHEVENOT, Julie
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorDE OLIVEIRA, Hugo
dc.contributor.authorPEREZ-ANDRES, Encarnacion
dc.contributor.authorBERRA, Edurne
dc.contributor.authorMASA, Marc
hal.structure.identifierGAIKER Technological Centre
dc.contributor.authorSUAREZ-MERINO, Blanca
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorLECOMMANDOUX, Sebastien
hal.structure.identifierGAIKER Technological Centre
dc.contributor.authorHEREDIA, Pedro
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2015
dc.identifier.issn1550-7033
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/20266
dc.description.abstractEnEncapsulating chemotherapy drugs in targeted nanodelivery systems is one of the most promising approaches to tackle cancer disease, avoiding side effects of common treatment. In the last decade, several nanocarriers with different nature have been tested, but polypeptide-based copolymers have attracted considerable attention for their biocompatibility, controlled and slow biodegradability as well as their low toxicity. In this work, we synthesized, characterized and evaluated poly(trimethylene carbonate) block poly(L-glutamic acid) derived polymersomes, targeted to epidermal growth factor receptor (EGFR), loaded with plitidepsin and ultimately tested in HT29 and LS174T colorectal cancer cell lines for specificity and efficacy. Furthermore, morphology, physico chemical properties and plitidepsin loading were carefully investigated. A thorough in vitro cytotoxicity analysis of the unloaded polymersomes was carried out for bioconnpatibility check, studying viability, cell membrane asymmetry and reactive oxygen species levels. Those cytotoxicity assays showed good biocompatibility for plitidepsin unloaded polymersomes. Cellular uptake and cytotoxic effect of EGFR targeted and plitidepsin loaded polymersome indicated that colorectal cancer cell lines were more sensitive to anti-EGFR-drug-loaded than untargeted drug-loaded polynnersomes. Also, in both cell lines, the use of untargeted polymersonnes greatly reduced plitidepsin cytotoxicity as well as the cellular uptake, indicating that the use of this targeted nanocarrier is a promising approach to tackle colorectal cancer disease and avoid the undesired effects of the usual treatment. Furthermore, in vivo assays support the in vitro conclusions that EGFR targeted polynnersomes could be a good drug delivery system. This work provides a proof of concept for the use of encapsulated targeted drugs as future therapeutic treatments for cancer.
dc.language.isoen
dc.publisherAmerican Scientific Publishers
dc.subject.enNANOPARTICLES
dc.subject.enDOWN-REGULATION
dc.subject.enBREAST-CANCER
dc.subject.enCETUXIMAB
dc.subject.enAmphiphilic Co-Polymers
dc.subject.enNanocarrier
dc.subject.enCancer Therapy
dc.subject.enPolymersome Uptake
dc.subject.enPlitidepsin
dc.subject.enANTI-EGFR ANTIBODY
dc.subject.enIN-VIVO
dc.subject.enBIODEGRADABLE POLYMERSOMES
dc.subject.enAPLIDIN
dc.subject.enEXPRESSION
dc.subject.enPACLITAXEL
dc.title.enCellular Uptake and Cytotoxic Effect of Epidermal Growth Factor Receptor Targeted and Plitidepsin Loaded Co-Polymeric Polymersomes on Colorectal Cancer Cell Lines
dc.typeArticle de revue
dc.identifier.doi10.1166/jbn.2015.2148
dc.subject.halChimie/Polymères
bordeaux.journalJournal of Biomedical Nanotechnology
bordeaux.page2034-2049
bordeaux.volume11
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue11
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-01361853
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01361853v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Journal%20of%20Biomedical%20Nanotechnology&rft.date=2015&rft.volume=11&rft.issue=11&rft.spage=2034-2049&rft.epage=2034-2049&rft.eissn=1550-7033&rft.issn=1550-7033&rft.au=GONI-DE-CERIO,%20Felipe&THEVENOT,%20Julie&DE%20OLIVEIRA,%20Hugo&PEREZ-ANDRES,%20Encarnacion&BERRA,%20Edurne&rft.genre=article


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