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Unraveling the mystery of natural rubber biosynthesis. PART II: Composition and growth of in vitro natural rubber using high-resolution size exclusion chromatography

dc.rights.licenseopen
hal.structure.identifierUniv Akron, Dept Polymer Sci
dc.contributor.authorCHIANG, Cheng Ching K.
hal.structure.identifierCenter for Nanophase Materials Sciences [Oak Ridge] [CNMS]
dc.contributor.authorBARKAKATY, Balaka
hal.structure.identifierUniv Akron, Dept Polymer Sci
hal.structure.identifierUniv Akron, Dept Chem & Biomol Engn
dc.contributor.authorPUSKAS, Judit E.
hal.structure.identifierOhio State Univ, OARDC
dc.contributor.authorXIE, Wenshuang
hal.structure.identifierOhio State Univ, OARDC
dc.contributor.authorCORNISH, Katrina
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 1 LCPO : Polymerization Catalyses & Engineering
dc.contributor.authorPERUCH, Frédéric
IDREF: 152900748
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 1 LCPO : Polymerization Catalyses & Engineering
dc.contributor.authorDEFFIEUX, Alain
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2014
dc.identifier.issn0035-9475
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/20231
dc.description.abstractEnThe superior properties of natural rubber (cis-1,4-polyisoprene [NR]) are a function of its structure and composition, properties that still remain a mystery and that are irreplaceable by any synthetic rubber. NR from guayule (Parthenium argentatum) has been gaining special interest for its hypoallergenic properties while maintaining superior mechanical properties that are commonly associated with the Brazilian rubber tree (Hevea brasiliensis), the most common source of NR. Techniques exist to isolate washed rubber particles (WRPs) that contain enzymatically active rubber transferase, to study NR biosynthesis, and previous work on the in vitro NR growth in Hevea has demonstrated the presence of around 50 wt% of a low molecular weight ([MW], M-n <10 000 g/mol) fraction. Structural and compositional analyses of this low MW fraction in Hevea are challenging due to the high protein content. We discuss the analysis and composition of guayule latex and WRPs using high-resolution Size Exclusion Chromatography. We also discuss the composition of the soluble fraction of inactive guayule latex using matrix-assisted laser desorption ionization/time of flight mass spectrometry.
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.subject.enPARTHENIUM-ARGENTATUM GRAY
dc.subject.enGUAYULE RUBBER
dc.title.enUnraveling the mystery of natural rubber biosynthesis. PART II: Composition and growth of in vitro natural rubber using high-resolution size exclusion chromatography
dc.typeArticle de revue
dc.identifier.doi10.5254/rct.14.87913
dc.subject.halChimie/Polymères
bordeaux.journalRubber Chemistry and Technology
bordeaux.page451-458
bordeaux.volume87
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue3
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-01368885
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01368885v1
bordeaux.COinSctx_ver=Z39.88-2004&amp;rft_val_fmt=info:ofi/fmt:kev:mtx:journal&amp;rft.jtitle=Rubber%20Chemistry%20and%20Technology&amp;rft.date=2014&amp;rft.volume=87&amp;rft.issue=3&amp;rft.spage=451-458&amp;rft.epage=451-458&amp;rft.eissn=0035-9475&amp;rft.issn=0035-9475&amp;rft.au=CHIANG,%20Cheng%20Ching%20K.&amp;BARKAKATY,%20Balaka&amp;PUSKAS,%20Judit%20E.&amp;XIE,%20Wenshuang&amp;CORNISH,%20Katrina&amp;rft.genre=article


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