Expression and purification of short hydrophobic elastin-like polypeptides with maltose-binding protein as a solubility tag
| dc.rights.license | open | |
| hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
| hal.structure.identifier | Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie [IECB] | |
| dc.contributor.author | BATAILLE, Laure | |
| hal.structure.identifier | Biotechnologie des protéines recombinantes à visée santé | |
| dc.contributor.author | DIERYCK, Wilfrid | |
| hal.structure.identifier | Biotechnologie des protéines recombinantes à visée santé | |
| dc.contributor.author | HOCQUELLET, Agnès | |
| hal.structure.identifier | Biotechnologie des protéines recombinantes à visée santé | |
| dc.contributor.author | CABANNE, Charlotte | |
| dc.contributor.author | BATHANY, Katell | |
| hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
| hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
| dc.contributor.author | LECOMMANDOUX, Sebastien | |
| hal.structure.identifier | Biotechnologie des protéines recombinantes à visée santé | |
| dc.contributor.author | GARBAY, Bertrand
IDREF: 033777551 | |
| hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
| hal.structure.identifier | Imagerie Moléculaire et Nanobiotechnologies - Institut Européen de Chimie et Biologie [IECB] | |
| hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
| dc.contributor.author | GARANGER, Elisabeth
IDREF: 089451740 | |
| dc.date.accessioned | 2020 | |
| dc.date.available | 2020 | |
| dc.date.issued | 2015 | |
| dc.identifier.issn | 1046-5928 | |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/20201 | |
| dc.description.abstractEn | Elastin-like polypeptides (ELPs) are biodegradable polymers with interesting physico-chemical properties for biomedical and biotechnological applications. The recombinant expression of hydrophobic elastin-like polypeptides is often difficult because they possess low transition temperatures, and therefore form aggregates at sub-ambient temperatures. To circumvent this difficulty, we expressed in Escherichia coli three hydrophobic ELPs (VPGIG)(n) with variable lengths (n = 20, 40, and 60) in fusion with the maltose-binding protein (MBP). Fusion proteins were soluble and yields of purified MBP-ELP ranged between 66 and 127 mg/L culture. After digestion of the fusion proteins by enterokinase, the ELF moiety was purified by using inverse transition cycling. The purified fraction containing ELP40 was slightly contaminated by traces of undigested fusion protein. Purification of ELP60 was impaired because of co-purification of the MBP tag during inverse transition cycling. ELP20 was successfully purified to homogeneity, as assessed by gel electrophoresis and mass spectrometry analyses. The transition temperature of ELP20 was measured at 15.4 degrees C in low salt buffer. In conclusion, this method can be used to produce hydrophobic ELF of low molecular mass | |
| dc.language.iso | en | |
| dc.publisher | Elsevier | |
| dc.subject.en | AQUEOUS-SOLUTION | |
| dc.subject.en | MOLECULAR-WEIGHT | |
| dc.subject.en | PHASE-TRANSITION | |
| dc.subject.en | Elastin-like polypeptides | |
| dc.subject.en | Precision polymers | |
| dc.subject.en | Recombinant expression | |
| dc.subject.en | Maltose-binding protein transition temperature | |
| dc.subject.en | Mass spectrometry | |
| dc.subject.en | INVERSE TEMPERATURE TRANSITION | |
| dc.subject.en | RECURSIVE DIRECTIONAL LIGATION | |
| dc.subject.en | ESCHERICHIA-COLI | |
| dc.subject.en | RECOMBINANT PROTEINS | |
| dc.subject.en | E. COLI | |
| dc.subject.en | MODEL | |
| dc.subject.en | FREE-ENERGY | |
| dc.title.en | Expression and purification of short hydrophobic elastin-like polypeptides with maltose-binding protein as a solubility tag | |
| dc.type | Article de revue | |
| dc.identifier.doi | 10.1016/j.pep.2015.03.013 | |
| dc.subject.hal | Chimie/Polymères | |
| bordeaux.journal | Protein Expression and Purification | |
| bordeaux.page | 165-171 | |
| bordeaux.volume | 110 | |
| bordeaux.hal.laboratories | Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629 | * |
| bordeaux.institution | Bordeaux INP | |
| bordeaux.institution | Université de Bordeaux | |
| bordeaux.peerReviewed | oui | |
| hal.identifier | hal-01372375 | |
| hal.version | 1 | |
| hal.origin.link | https://hal.archives-ouvertes.fr//hal-01372375v1 | |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Protein%20Expression%20and%20Purification&rft.date=2015&rft.volume=110&rft.spage=165-171&rft.epage=165-171&rft.eissn=1046-5928&rft.issn=1046-5928&rft.au=BATAILLE,%20Laure&DIERYCK,%20Wilfrid&HOCQUELLET,%20Agn%C3%A8s&CABANNE,%20Charlotte&BATHANY,%20Katell&rft.genre=article |
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