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Biocompatible Polyion Complex Micelles Synthesized from Arborescent Polymers

dc.rights.licenseopen
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierLaboratory of Mammalian Cell Culture [GIGA-R]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
hal.structure.identifierDepartment of Chemistry [Waterloo]
dc.contributor.authorNGUYEN, Vo Thu An
hal.structure.identifierLaboratory of Mammalian Cell Culture [GIGA-R]
dc.contributor.authorDE PAUW-GILLET, Marie-Claire
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorSANDRE, Olivier
hal.structure.identifierDepartment of Chemistry [Waterloo]
dc.contributor.authorGAUTHIER, Mario
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2016
dc.identifier.issn0743-7463
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/20131
dc.description.abstractEnWater-dispersible polyion complex (PIC) micelles were prepared by the self-assembly of an arborescent polystyrene-graft-poly(2-vinylpyridine) copolymer (denoted G0PS-g-P2VP or G1) serving as core and a poly(acrylic acid)-block-poly(2-hydroxyethyl acrylate) (PAA-b-PHEA) double-hydrophilic block copolymer (DHBC) forming a shell. Varying the density of hydrophilic polymer chains in the stabilizing layer provided control over the size and structure of the entities obtained, from large flocculated species to stable isolated PIC micelles with diameters ranging from 42 to 67 nm. The hydrodynamic radius (determined from dynamic light scattering measurements), and the weight-average molar mass (M̅w) and radius of gyration of the scatterers (extracted from static multi-angle light scattering data) evidenced the formation of either isolated or aggregated PIC micelles depending on the self-assembly conditions used (pH, concentration and mixing molar ratio f). Changes in the morphology of the arborescent copolymer after complexation were observed by atomic force microscopy (AFM) imaging. In particular, by varying the force applied with the AFM tip on the samples, the core–shell structure of the PIC micelles was clearly evidenced. The PIC micelles displayed no significant cytotoxicity towards mouse fibroblast L929 cells, a standard cell line recommended for toxicity assays, due to the good biocompatibility of the hydrophilic PAA-b-PHEA shell. In spite of a negative residual zeta potential due to an excess of negative charges, fluorescently labeled PIC* micelles were successful internalized by L929 cells, as confirmed by laser scanning confocal microscopy (LSCM) and transmission electron microscopy (TEM).
dc.description.sponsorshipMagnéto-Chimiothérapie : Modélisation de la Délivrance Induite par Champ Magnétique Radiofréquence d'Anticancéreux par des Nano-Vésicules Polymères et Suivi par IRM d'un Modèle de Glioblastome - ANR-13-BS08-0017
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/
dc.subject.enPolyion complex micelles
dc.subject.enDouble hydrophilic block copolymers
dc.subject.enArborescent copolymers
dc.subject.enStealth properties
dc.subject.enIn vitro assay
dc.title.enBiocompatible Polyion Complex Micelles Synthesized from Arborescent Polymers
dc.typeArticle de revue
dc.identifier.doi10.1021/acs.langmuir.6b03683
dc.subject.halChimie/Polymères
dc.subject.halChimie/Matériaux
bordeaux.journalLangmuir
bordeaux.page13482-13492
bordeaux.volume32
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue50
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-01404628
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01404628v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Langmuir&rft.date=2016&rft.volume=32&rft.issue=50&rft.spage=13482-13492&rft.epage=13482-13492&rft.eissn=0743-7463&rft.issn=0743-7463&rft.au=NGUYEN,%20Vo%20Thu%20An&DE%20PAUW-GILLET,%20Marie-Claire&SANDRE,%20Olivier&GAUTHIER,%20Mario&rft.genre=article


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