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Membrane properties of giant polymer and lipid vesicles obtained by electroformation and PVA gel-assisted hydration methods

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hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorDAO, Thi Phuong Tuyen
dc.contributor.authorFAUQUIGNON, Martin
hal.structure.identifierUniv Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol
hal.structure.identifierUniv Lisbon, Inst Super Tecn, Ctr Quim Fis Mol
dc.contributor.authorFERNANDES, Fabio
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorIBARBOURE, Emmanuel
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
dc.contributor.authorVAX, Amélie
hal.structure.identifierUniv Lisbon, Inst Super Tecn, Ctr Quim Fis Mol
hal.structure.identifierUniv Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol
dc.contributor.authorPRIETO, Manuel
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
dc.contributor.authorLE MEINS, Jean-Francois
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2017
dc.identifier.issn0927-7757
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/20101
dc.description.abstractEnGiant Unilamellar Vesicles (GUV) are the most commonly used biomimetic membrane model. Their production in physiological conditions through the well-known electroformation process is complex and other gel-assisted hydration procedures have recently emerged to circumvent some limitations of the process. In this work, the membrane physical properties of Giant Polymer Unilamellar Vesicles (Polymersomes) and lipid vesicles (Liposomes) obtained from the gel-assisted hydration procedure and electroformation, have been evaluated thoroughly by different techniques: micropipettes aspiration (MPA), Fluorescence Recovery After Photo Bleaching (FRAP) and Laurdan Generalized Polarization (GP). The study was performed on GUVs made of either phospholipids (POPC and DPPC) or amphiphilic copolymers based on Poly(dimethylsiloxane) and poly(ethyleneoxide). A significant deviation of stretching modulus, lateral diffusion coefficient and membrane packing was observed for GUVs formed by the PVA gel-assisted hydration method compared to the classical electroformation process. We were able to show that this deviation was due to the presence of PVA chains in the membrane and in the suspending medium. Globally our findings reveal that particular attention has to be paid on the gel assisted hydration process as the vesicles obtained present altered mechanical behaviour.
dc.description.sponsorshipCinétique de Translocation de Particules à travers de Bicouches Auto-Assemblées - ANR-12-BS08-0018
dc.language.isoen
dc.publisherElsevier
dc.subject.enLipid
dc.subject.enVesicle
dc.subject.enElectroformation
dc.subject.enmembrane properties
dc.subject.enpolymer
dc.subject.enGel hydration
dc.title.enMembrane properties of giant polymer and lipid vesicles obtained by electroformation and PVA gel-assisted hydration methods
dc.typeArticle de revue
dc.identifier.doi10.1016/j.colsurfa.2017.09.005
dc.subject.halPhysique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft]
dc.subject.halChimie/Matériaux
dc.description.sponsorshipEuropeErasmus Mundus - International Doctoral School in Functional Materials
bordeaux.journalColloids and Surfaces A: Physicochemical and Engineering Aspects
bordeaux.page347-353
bordeaux.volume553
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-01585779
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01585779v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Colloids%20and%20Surfaces%20A:%20Physicochemical%20and%20Engineering%20Aspects&rft.date=2017&rft.volume=553&rft.spage=347-353&rft.epage=347-353&rft.eissn=0927-7757&rft.issn=0927-7757&rft.au=DAO,%20Thi%20Phuong%20Tuyen&FAUQUIGNON,%20Martin&FERNANDES,%20Fabio&IBARBOURE,%20Emmanuel&VAX,%20Am%C3%A9lie&rft.genre=article


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