Optimization of extraction and development of an LC-HRMS method to quantify glutathione and glutathione disulfide in white wine lees and yeast derivatives
Idioma
EN
Article de revue
Este ítem está publicado en
Food Chemistry. 2024-05-01, vol. 439, p. 138121
Resumen en inglés
The antioxidant capacity of wine depends on its quality and aging potential. Aging on lees can improve this capacity thanks to the release of glutathione (GSH), as can the addition of yeast derivatives (YD). Therefore, the ...Leer más >
The antioxidant capacity of wine depends on its quality and aging potential. Aging on lees can improve this capacity thanks to the release of glutathione (GSH), as can the addition of yeast derivatives (YD). Therefore, the GSH potential of wine lees (WL) and YD requires investigation. We propose an optimized method to extract and quantify GSH from WL and YD. First, a method was developed to detect and quantify GSH and glutathione disulfide (GSSG) using LC-HRMS. Second, Box-Behnken response surface methodologies (RSM) were applied to both matrices. Results showed that the main parameter affecting GSH extraction efficiency was ethanol concentration. Quantitation of various samples revealed GSH concentrations of up to 900 μg/g for WL and 40 mg/g for YD. To our knowledge, the absolute quantitation of GSH/GSSG in these matrices has not been reported until now.< Leer menos
Palabras clave en inglés
Glutathione
Glutathione disulfide
Wine lees
RSM design
LC-HRMS
Proyecto ANR
Valorisation de lies de vin par procédés eco-responsables pour l'obtention de produits œnologiques. - ANR-21-CE43-0003
Centros de investigación