BAILLEUL, Alexia; DE GANNES, Florence Poulletier; PIROG, Antoine; N’KAOUA, Gilles; D’HOLLANDE, Adrien; HOUE, Aurelian; SOULIER, Fabien; BERNARD, Serge; RENAUD, Sylvie

doi:10.1109/ACCESS.2022.3228479

Laboratoire de l'intégration, du matériau au système [IMS]

DE GANNES, Florence Poulletier
Laboratoire de l'intégration, du matériau au système [IMS]

PIROG, Antoine
Laboratoire de l'intégration, du matériau au système [IMS]

Langue

Article de revue

Ce document a été publié dans

IEEE Access. 2022-12-12, vol. 10, p. 131430-131441

Résumé en anglais

In this study, we present a first-of-a-kind biological-hardware-software tool to evaluate the physiological condition of in vitro myotubes in response to electrical stimulation. We demonstrate that impedance spectroscopy on a microelectrode array can testify for physiological changes of muscle cells under electrical stimulation. The platform is designed for simultaneous bioimpedance spectroscopy, electrical stimulation, and optical microscopy. It includes a microelectrode array, a custom hardware-software interface, and a commercial impedance analyzer. We used a well-established muscle cell model (C2C12) and developed a culture protocol suited for long-term recordings on microelectrode arrays. Electrical stimulation was applied with carbon electrodes and ad hoc electronics for current stimulation. Muscle cell bioimpedance measurement was complemented with optical microscopy video to record contractions. Then, the influence of electrical stimulation on the contractile activity of myotubes and on their bioimpedance was analyzed. Results validated the functionality of the hardware/software platform when used with our contractile muscle model. A bioimpedance-based metric was defined to evaluate changes in myotubes’ physiology. After playing multiple stimulation scenarios, analysis showed that the bioimpedance metric decreases as duration or frequency of stimulation increase.< Réduire

Mots clés

Bioimpedance

Muscles

Electrical stimulation

Monitoring

Impedance

Spectroscopy

In vitro

C2C12

Electrical stimulation

Electrophysiology

Impedance spectroscopy

MEA

Microelectrode array

Myotubes

Skeletal muscle

URI

https://oskar-bordeaux.fr/handle/20.500.12278/199959

DOI

http://dx.doi.org/10.1109/ACCESS.2022.3228479

Project ANR

Capteurs bio-électroniques intégrant l'algorithme des îlots pour le contrôle de la glycémie en boucle ouverte et fermée - ANR-18-CE17-0005

Unités de recherche

IMS : Laboratoire de l'Intégration du Matériau au Système - UMR 5218

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Bioimpedance Spectroscopy Helps Monitor the Impact of Electrical Stimulation on Muscle Cells