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Modulation of phase separation at micron scale and nanoscale in hybrid polymer/lipid giant unilamellar vesicles (GHUVs)

dc.rights.licenseopen
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorDAO, Tuyen P. T.
hal.structure.identifierUniv Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol
hal.structure.identifierUniv Lisbon, Inst Super Tecn, Ctr Quim Fis Mol
dc.contributor.authorFERNANDES, Fabio
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorIBARBOURE, Emmanuel
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorFERJI, Khalid
hal.structure.identifierUniv Lisbon, Inst Super Tecn, Ctr Quim Fis Mol
hal.structure.identifierUniv Lisbon, Inst Super Tecn, Inst Nanosci & Nanotechnol
dc.contributor.authorPRIETO, Manuel
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
dc.contributor.authorSANDRE, Olivier
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorLE MEINS, Jean-Francois
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2017
dc.identifier.issn1744-683X
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/19959
dc.description.abstractEnPhase separation in hybrid polymer/lipid giant unillamelar vesicles (GHUVs) has been described over the last few years. However there is still a lack of understanding in physical and molecular factors governing the phase separation in such systems. Among these parameters it has been suggested that in analogy to multicomponent lipid vesicles that hydrophobic mismatchs as well as lipid fludity play a role.In this work, we aim to map a global picture of phase separation and domain formation in membrane of GHUVs by using various copolymers based on poly(dimethylsiloxane) (PDMS) and poly(ethylene glycol) (PEO) with different architectures (grafted, triblock) and molar masses, combined with lipid at fluid (POPC) or gel state (DPPC) at room temperature. From confocal imaging and fluorescence lifetime imaging microscopy technique (FLIM), the phase separation into either micro- or nano- domains within GHVs were studied. In particular, our systematic studies demonstrate that in addition to lipid/polymer fraction or lipid physical state, important factors such as line tension at lipid polymer/lipid boundaries can be finely modulated by the molar mass and architecture of the copolymer and lead to the formation of stable lipid domains with different sizes and morphologies in such GHUVs.
dc.description.sponsorshipCinétique de Translocation de Particules à travers de Bicouches Auto-Assemblées - ANR-12-BS08-0018
dc.language.isoen
dc.publisherRoyal Society of Chemistry
dc.subject.enCryo Electron Microscopy
dc.subject.enHybrid lipid polymer vesicles
dc.subject.enNanodomains
dc.subject.enNeutron scattering including small-angle scattering
dc.subject.enMembranes bilayers and vesicles
dc.subject.enFluorescence resonance energy transfer FRET
dc.title.enModulation of phase separation at micron scale and nanoscale in hybrid polymer/lipid giant unilamellar vesicles (GHUVs)
dc.typeArticle de revue
dc.identifier.doi10.1039/C6SM01625A
dc.subject.halChimie/Polymères
bordeaux.journalSoft Matter
bordeaux.page627-637
bordeaux.volume13
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.issue3
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-01417534
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-01417534v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Soft%20Matter&rft.date=2017&rft.volume=13&rft.issue=3&rft.spage=627-637&rft.epage=627-637&rft.eissn=1744-683X&rft.issn=1744-683X&rft.au=DAO,%20Tuyen%20P.%20T.&FERNANDES,%20Fabio&IBARBOURE,%20Emmanuel&FERJI,%20Khalid&PRIETO,%20Manuel&rft.genre=article


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