DAVIDSON, Sean; BOULANGER, Chantal; AIKAWA, Elena; BADIMON, Lina; BARILE, Lucio; BINDER, Christoph; BRISSON, Alain; BUZAS, Edit; EMANUELI, Costanza; JANSEN, Felix; KATSUR, Miroslava; LACROIX, Romaric; LIM, Sai; MACKMAN, Nigel; MAYR, Manuel; MENASCHE, Philippe; NIEUWLAND, Rienk; SAHOO, Susmita; TAKOV, Kaloyan; THUM, Thomas; VADER, Pieter; WAUBEN, Marca; WITWER, Kenneth; SLUIJTER, Joost

doi:10.1093/cvr/cvac031

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Article de revue

Ce document a été publié dans

Cardiovascular Research. 2023, vol. 1149, n° 1, p. 45-63

Résumé en anglais

Extracellular vesicles (EVs) are nanosized vesicles with a lipid bilayer that are released from cells of the cardiovascular system, and are considered important mediators of intercellular and extracellular communication. Two types of EV of particular interest are exosomes and microvesicles, which have been identified in all tissue and body fluids and carry a variety of molecules including RNAs, proteins, and lipids. EVs have potential for use in the diagnosis and prognosis of cardiovascular diseases and as new therapeutic agents, particularly in the setting of myocardial infarction and heart failure. Despite their promise, technical challenges related to their small size make it challenging to accurately identify and characterize them, and to study EV-mediated processes. Here, we aim to provide the reader with an overview of the techniques and technologies available for the separation and characterization of EVs from different sources. Methods for determining the protein, RNA and lipid content of EVs are discussed. The aim of this document is to provide guidance on critical methodological issues and highlight key points for consideration for the investigation of EVs in cardiovascular studies.< Réduire

Mots clés en anglais

cardiovascular diseases

exosomes

extracellular vesicles

micro vesicles

plasma

URI

https://oskar-bordeaux.fr/handle/20.500.12278/199585

DOI

http://dx.doi.org/10.1093/cvr/cvac031

Project ANR

ARN non codant d'origine cardiomyocytaire et remodelage cardiaque post-ischémique - ANR-16-CE92-0032

Unités de recherche

CBMN : Chimie & de Biologie des Membranes & des Nano-objets - UMR 5248