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dc.rights.licenseopenen_US
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorGIRAULT, Mathias
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorBENEYTON, Thomas
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorPEKIN, Deniz
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorBUISSON, Lionel
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorBICHON, Sabrina
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorCHARBONNIER, Celine
IDREF: 176318933
hal.structure.identifierEnvironnements et Paléoenvironnements OCéaniques [EPOC]
dc.contributor.authorDEL AMO, Yolanda
hal.structure.identifierCentre de Recherche Paul Pascal [CRPP]
dc.contributor.authorBARET, Jean-Christophe
IDREF: 19204690X
dc.date.accessioned2024-04-26T11:04:02Z
dc.date.available2024-04-26T11:04:02Z
dc.date.issued2018
dc.identifier.issn0003-2700en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/199353
dc.description.abstractEnOne way for phytoplankton to survive orthophosphate depletion is to utilize dissolved organic phosphorus by expressing alkaline phosphatase. The actual methods to assay alkaline phosphate activity—either in bulk or as a presence/absence of enzyme activity—fail to provide information on individual living cells. In this context, we develop a new microfluidic method to compartmentalize cells in 0.5 nL water-in-oil droplets and measure alkaline phosphatase activity at the single-cell level. We use enzyme-labeled fluorescence (ELF), which is based on the hydrolysis of ELF-P substrate, to monitor in real time and at the single-cell level both qualitative and quantitative information on cell physiology (i.e., localization and number of active enzyme sites and alkaline phosphatase kinetics). We assay the alkaline phosphatase activity of Tetraselmis sp. as a function of the dissolved inorganic phosphorus concentration and show that the time scale of the kinetics spans 1 order of magnitude. The advantages of subnanoliter-scale compartmentalization in droplet-based microfluidics provide a precise characterization of a population with single-cell resolution. Our results highlight the key role of cell physiology to efficiently access dissolved organic phosphorus.
dc.description.sponsorshipInitiative d'excellence de l'Université de Bordeaux - ANR-10-IDEX-0003en_US
dc.language.isoENen_US
dc.subject.enMicrofluic
dc.subject.enVesicles
dc.subject.enDroplet
dc.title.enHigh-Content Screening of Plankton Alkaline Phosphatase Activity in Microfluidics
dc.typeArticle de revueen_US
dc.identifier.doi10.1021/acs.analchem.8b00234en_US
dc.subject.halSciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire/Biochimie [q-bio.BM]en_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologieen_US
dc.subject.halSciences de l'environnement/Ingénierie de l'environnementen_US
bordeaux.journalAnalytical Chemistryen_US
bordeaux.page4174 - 4181en_US
bordeaux.volume90en_US
bordeaux.hal.laboratoriesEPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805en_US
bordeaux.issue6en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionCNRSen_US
bordeaux.teamECOBIOCen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-01757331
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Analytical%20Chemistry&rft.date=2018&rft.volume=90&rft.issue=6&rft.spage=4174%20-%204181&rft.epage=4174%20-%204181&rft.eissn=0003-2700&rft.issn=0003-2700&rft.au=GIRAULT,%20Mathias&BENEYTON,%20Thomas&PEKIN,%20Deniz&BUISSON,%20Lionel&BICHON,%20Sabrina&rft.genre=article


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