The tip protein PAAR is required for the function of the type VI secretion system
| dc.rights.license | open | en_US |
| hal.structure.identifier | Laboratoire d'ingénierie des systèmes macromoléculaires [LISM] | |
| dc.contributor.author | BEAUVOIS, Solène | |
| hal.structure.identifier | Laboratoire d'ingénierie des systèmes macromoléculaires [LISM] | |
| dc.contributor.author | FLAUGNATTI, Nicolas | |
| hal.structure.identifier | Bioénergétique et Ingénierie des Protéines [BIP ] | |
| dc.contributor.author | ILBERT, Marianne | |
| hal.structure.identifier | Laboratoire d'ingénierie des systèmes macromoléculaires [LISM] | |
| dc.contributor.author | BOYER, Marie | |
| hal.structure.identifier | Institut Européen de Chimie et Biologie [IECB] | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | GAVELLO-FERNANDEZ, Esther | |
| hal.structure.identifier | Institut Européen de Chimie et Biologie [IECB] | |
| hal.structure.identifier | Microbiologie Fondamentale et Pathogénicité [MFP] | |
| dc.contributor.author | FRONZES, Rémi | |
| hal.structure.identifier | Laboratoire d'ingénierie des systèmes macromoléculaires [LISM] | |
| dc.contributor.author | JURĖNAS, Dukas | |
| hal.structure.identifier | Laboratoire d'ingénierie des systèmes macromoléculaires [LISM] | |
| dc.contributor.author | JOURNET, Laure | |
| dc.date.accessioned | 2024-04-24T11:37:20Z | |
| dc.date.available | 2024-04-24T11:37:20Z | |
| dc.date.issued | 2023-10-06 | |
| dc.identifier.issn | 2165-0497 | en_US |
| dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/199313 | |
| dc.description.abstractEn | Bacteria are constantly competing to colonize crowded ecological niches, such as the human gut. The type VI secretion system (T6SS) is a critical bacterial weapon in this warfare. It resembles a crossbow with a poisoned arrow allowing bacteria to inject toxic effectors directly into target cells. This machinery is formed by an envelopespanning complex which recruits the baseplate, an assembly platform allowing the polymerization of a contractile structure. The tail consists of a tube surrounded by a sheath and topped by the needle complex composed of the VgrG and PAAR proteins. In the enteric pathogen enteroaggregative Escherichia coli (EAEC), the Tle1 phospholipase toxin ensures the antibacterial activity of the T6SS-1. For its transport, Tle1 interacts directly with the trimeric spike protein VgrG. However, the importance and the function of the tip protein PAAR in the T6SS remain unclear. Here, we characterized the PAAR protein of EAEC using biochemical, fluorescence microscopy, and antibacterial competi tion approaches. Using pull-down assays and cryo-electron microscopy analysis of the (VgrG) 3-(Tle1) 3-PAAR complex, we show that PAAR tops and closes the β-prism structure of the VgrG spike. The PAAR protein structure is further tightened by the zinc atom coordinated via conserved residues essential for its function. We provide evidence that PAAR is necessary for T6SS-1-mediated killing due to its requirement for proper T6SS baseplate assembly and further sheath polymerization. Our results suggest that the PAAR protein is an essential component of T6SS. | |
| dc.description.sponsorship | Contribution des Effecteurs du Système de Sécrétion de Type VI à la pathogénicité des Escherichia coli adhérents-invasifs - ANR-18-CE15-0013 | en_US |
| dc.language.iso | EN | en_US |
| dc.rights.uri | http://creativecommons.org/licenses/by/ | |
| dc.subject.en | Type VI secretion system | |
| dc.subject.en | cryo-electron microscopy | |
| dc.subject.en | bacterial competition | |
| dc.subject.en | protein secretion | |
| dc.subject.en | VgrG | |
| dc.subject.en | bacterial toxin | |
| dc.subject.en | zinc | |
| dc.title.en | The tip protein PAAR is required for the function of the type VI secretion system | |
| dc.type | Article de revue | en_US |
| dc.identifier.doi | 10.1128/spectrum.01478-23 | en_US |
| dc.subject.hal | Sciences du Vivant [q-bio]/Biochimie, Biologie Moléculaire | en_US |
| dc.identifier.pubmed | 37800964 | en_US |
| bordeaux.journal | Microbiology Spectrum | en_US |
| bordeaux.hal.laboratories | MFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234 | en_US |
| bordeaux.institution | CNRS | en_US |
| bordeaux.peerReviewed | oui | en_US |
| bordeaux.inpress | non | en_US |
| bordeaux.import.source | hal | |
| hal.identifier | hal-04260438 | |
| hal.version | 1 | |
| hal.popular | non | en_US |
| hal.audience | Internationale | en_US |
| hal.export | false | |
| workflow.import.source | hal | |
| dc.rights.cc | Pas de Licence CC | en_US |
| bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Microbiology%20Spectrum&rft.date=2023-10-06&rft.eissn=2165-0497&rft.issn=2165-0497&rft.au=BEAUVOIS,%20Sol%C3%A8ne&FLAUGNATTI,%20Nicolas&ILBERT,%20Marianne&BOYER,%20Marie&GAVELLO-FERNANDEZ,%20Esther&rft.genre=article |
