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Identification of a novel and ancestral machinery involved in mitochondrial membrane branching in Trypanosoma brucei

dc.rights.licenseopenen_US
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorMOREL, Chloé Alexandra
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorASENCIO, Corinne
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorBLANCARD, Corinne
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorSALIN, Bénédicte
hal.structure.identifierBordeaux Imaging Center [BIC]
dc.contributor.authorGONTIER, Etienne
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorDUVEZIN-CAUBET, Stéphane
hal.structure.identifierInstitut de biochimie et génétique cellulaires [IBGC]
dc.contributor.authorROJO, Manuel
hal.structure.identifierUniversité de Bordeaux [UB]
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBRINGAUD, Frédéric
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorTETAUD, Emmanuel
IDREF: 25436697X
dc.date.accessioned2024-04-23T13:40:58Z
dc.date.available2024-04-23T13:40:58Z
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/199288
dc.description.abstractEnABSTRACT African trypanosomes are eukaryotic parasites that exist in two main replicative forms; the procyclic form in the midgut of the insect vector, the tsetse fly Glossina spp. and the bloodstream form responsible for diseases in humans and cattle. Unlike most other eukaryotes, where mitochondria continuously fuse and divide, trypanosome mitochondria form a single and continuously interconnected network that only divides during cytokinesis. The machineries governing mitochondrial remodeling and interconnection, however, remain largely unknown. We characterize a dynamin-related protein (DRP) from T. brucei ( Tb DBF, previously called Tb MfnL) that depicts sequence similarities with Opa1 and Mfn, mammalian DRPs involved mitochondrial fusion. We showed that Tb DBF has closely related homologues in several organisms that are devoid of Mfn and Opa1, such as eukaryotes from different phyla, prokaryotes and archaea. Tb DBF is the first member of this new protein family to be functionally characterized. It localizes to the mitochondrial periphery and, upon overexpression, induces a strong increase in the interconnection and branching of mitochondrial filaments in a GTPase dependent manner. Its overexpression also promotes a major increase in cellular and mitochondrial volume and an increased consumption of the two major carbon sources used by the parasite (glucose and proline), as well as ethanolamine, a precursor of phosphatidyl-ethanolamine involved in membrane biogenesis and shaping. We propose that mitochondrial Tb DBF is a component of an ancestral membrane remodeling machinery that contributes to the formation of intermitochondrial connections.
dc.language.isoENen_US
dc.title.enIdentification of a novel and ancestral machinery involved in mitochondrial membrane branching in Trypanosoma brucei
dc.typeDocument de travail - Pré-publicationen_US
dc.identifier.doi10.1101/2023.06.28.546890en_US
dc.subject.halSciences du Vivant [q-bio]en_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologieen_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.institutionCNRSen_US
bordeaux.import.sourcehal
hal.identifierhal-04300070
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.subtypePrepublication/Preprinten_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.au=MOREL,%20Chlo%C3%A9%20Alexandra&ASENCIO,%20Corinne&BLANCARD,%20Corinne&SALIN,%20B%C3%A9n%C3%A9dicte&GONTIER,%20Etienne&rft.genre=preprint


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