Methods for the identification and characterization of extracellular vesicles in cardiovascular studies: from exosomes to microvesicles
Langue
EN
Article de revue
Ce document a été publié dans
Cardiovascular Research. 2023-01-01, vol. 119, n° 1, p. 45-63
Résumé en anglais
Extracellular vesicles (EVs) are nanosized vesicles with a lipid bilayer that are released from cells of the cardiovascular system, and are considered important mediators of intercellular and extracellular communications. ...Lire la suite >
Extracellular vesicles (EVs) are nanosized vesicles with a lipid bilayer that are released from cells of the cardiovascular system, and are considered important mediators of intercellular and extracellular communications. Two types of EVs of particular interest are exosomes and microvesicles, which have been identified in all tissue and body fluids and carry a variety of molecules including RNAs, proteins, and lipids. EVs have potential for use in the diagnosis and prognosis of cardiovascular diseases and as new therapeutic agents, particularly in the setting of myocardial infarction and heart failure. Despite their promise, technical challenges related to their small size make it challenging to accurately identify and characterize them, and to study EV-mediated processes. Here, we aim to provide the reader with an overview of the techniques and technologies available for the separation and characterization of EVs from different sources. Methods for determining the protein, RNA, and lipid content of EVs are discussed. The aim of this document is to provide guidance on critical methodological issues and highlight key points for consideration for the investigation of EVs in cardiovascular studies.< Réduire
Mots clés en anglais
Cardiovascular diseases
Exosomes
Extracellular vesicles
Micro vesicles
Plasma
Project ANR
ARN non codant d'origine cardiomyocytaire et remodelage cardiaque post-ischémique
Unités de recherche