dc.rights.license | open | |
hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
dc.contributor.author | GRAZON, Chloé | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | National Emerging Infectious Diseases Laboratories | |
dc.contributor.author | BAER, R. | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Department of Biomedical Engineering [Boston] | |
dc.contributor.author | KUZMANOVIĆ, Uroš | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Department of Biomedical Engineering [Boston] | |
dc.contributor.author | NGUYEN, Thuy | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Department of Biomedical Engineering [Boston] | |
dc.contributor.author | CHEN, Mingfu | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Department of Biomedical Engineering [Boston] | |
dc.contributor.author | ZAMANI, Marjon | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Division of Materials Science and Engineering | |
dc.contributor.author | CHERN, Margaret | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Department of Biomedical Engineering [Boston] | |
dc.contributor.author | AQUINO, Patricia | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Department of Biomedical Engineering [Boston] | |
dc.contributor.author | ZHANG, Xiaoman | |
hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
dc.contributor.author | LECOMMANDOUX, Sebastien | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Department of Biomedical Engineering [Boston] | |
dc.contributor.author | FAN, Andy | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Department of Biomedical Engineering [Boston] | |
dc.contributor.author | CABODI, Mario | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Department of Biomedical Engineering [Boston] | |
dc.contributor.author | KLAPPERICH, Catherine | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Department of Biomedical Engineering [Boston] | |
dc.contributor.author | GRINSTAFF, Mark | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Department of Biomedical Engineering [Boston] | |
dc.contributor.author | DENNIS, Allison | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Department of Biomedical Engineering [Boston] | |
dc.contributor.author | GALAGAN, James | |
dc.date.accessioned | 2020 | |
dc.date.available | 2020 | |
dc.date.issued | 2020 | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/19719 | |
dc.description.abstractEn | Bacteria are an enormous and largely untapped reservoir of biosensing proteins. We describe an approach to identify and isolate bacterial allosteric transcription factors (aTFs) that recognize a target analyte and to develop these TFs into biosensor devices. Our approach utilizes a combination of genomic screens and functional assays to identify and isolate biosensing TFs, and a quantum-dot Förster Resonance Energy Transfer (FRET) strategy for transducing analyte recognition into real-time quantitative measurements. We use this approach to identify a progesterone-sensing bacterial aTF and to develop this TF into an optical sensor for progesterone. The sensor detects progesterone in artificial urine with sufficient sensitivity and specificity for clinical use, while being compatible with an inexpensive and portable electronic reader for point-of-care applications. Our results provide proof-of-concept for a paradigm of microbially-derived biosensors adaptable to inexpensive, real-time sensor devices. | |
dc.language.iso | en | |
dc.publisher | Nature Publishing Group | |
dc.rights.uri | http://creativecommons.org/licenses/by/ | |
dc.subject.en | Bioassays | |
dc.title.en | A progesterone biosensor derived from microbial screening | |
dc.type | Article de revue | |
dc.identifier.doi | 10.1038/s41467-020-14942-5 | |
dc.subject.hal | Sciences du Vivant [q-bio]/Biotechnologies | |
dc.subject.hal | Sciences du Vivant [q-bio]/Médecine humaine et pathologie/Endocrinologie et métabolisme | |
dc.subject.hal | Chimie/Polymères | |
dc.subject.hal | Sciences du Vivant [q-bio]/Ingénierie biomédicale/Biomatériaux | |
dc.description.sponsorshipEurope | A Wearable Sensor for Hormones Based on a Native Microbial Sensing | |
bordeaux.journal | Nature Communications | |
bordeaux.page | 1276 | |
bordeaux.volume | 11 | |
bordeaux.hal.laboratories | Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629 | * |
bordeaux.institution | Bordeaux INP | |
bordeaux.institution | Université de Bordeaux | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-02501228 | |
hal.version | 1 | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-02501228v1 | |
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