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hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorGRAZON, Chloé
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierNational Emerging Infectious Diseases Laboratories
dc.contributor.authorBAER, R.
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorKUZMANOVIĆ, Uroš
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorNGUYEN, Thuy
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorCHEN, Mingfu
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorZAMANI, Marjon
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDivision of Materials Science and Engineering
dc.contributor.authorCHERN, Margaret
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorAQUINO, Patricia
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorZHANG, Xiaoman
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorLECOMMANDOUX, Sebastien
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorFAN, Andy
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorCABODI, Mario
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorKLAPPERICH, Catherine
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorGRINSTAFF, Mark
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorDENNIS, Allison
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorGALAGAN, James
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2020
dc.identifier.issn2041-1723
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/19719
dc.description.abstractEnBacteria are an enormous and largely untapped reservoir of biosensing proteins. We describe an approach to identify and isolate bacterial allosteric transcription factors (aTFs) that recognize a target analyte and to develop these TFs into biosensor devices. Our approach utilizes a combination of genomic screens and functional assays to identify and isolate biosensing TFs, and a quantum-dot Förster Resonance Energy Transfer (FRET) strategy for transducing analyte recognition into real-time quantitative measurements. We use this approach to identify a progesterone-sensing bacterial aTF and to develop this TF into an optical sensor for progesterone. The sensor detects progesterone in artificial urine with sufficient sensitivity and specificity for clinical use, while being compatible with an inexpensive and portable electronic reader for point-of-care applications. Our results provide proof-of-concept for a paradigm of microbially-derived biosensors adaptable to inexpensive, real-time sensor devices.
dc.language.isoen
dc.publisherNature Publishing Group
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enBioassays
dc.title.enA progesterone biosensor derived from microbial screening
dc.typeArticle de revue
dc.identifier.doi10.1038/s41467-020-14942-5
dc.subject.halSciences du Vivant [q-bio]/Biotechnologies
dc.subject.halSciences du Vivant [q-bio]/Médecine humaine et pathologie/Endocrinologie et métabolisme
dc.subject.halChimie/Polymères
dc.subject.halSciences du Vivant [q-bio]/Ingénierie biomédicale/Biomatériaux
dc.description.sponsorshipEuropeA Wearable Sensor for Hormones Based on a Native Microbial Sensing
bordeaux.journalNature Communications
bordeaux.page1276
bordeaux.volume11
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.peerReviewedoui
hal.identifierhal-02501228
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02501228v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Nature%20Communications&rft.date=2020&rft.volume=11&rft.spage=1276&rft.epage=1276&rft.eissn=2041-1723&rft.issn=2041-1723&rft.au=GRAZON,%20Chlo%C3%A9&BAER,%20R.&KUZMANOVI%C4%86,%20Uro%C5%A1&NGUYEN,%20Thuy&CHEN,%20Mingfu&rft.genre=article


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