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QD-FRET-based biosensing of small molecule analytes using transcription factor-DNA binding

dc.rights.licenseopen
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorNGUYEN, Thuy T.
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorCHERN, Margaret
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierNational Emerging Infectious Diseases Laboratories [NEIDL]
dc.contributor.authorBAER, R. C.
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorFAN, Andy
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Chemistry, Boston University
hal.structure.identifierLaboratoire de Chimie des Polymères Organiques [LCPO]
hal.structure.identifierTeam 3 LCPO : Polymer Self-Assembly & Life Sciences
dc.contributor.authorGRAZON, Chloé
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorGALAGAN, James
hal.structure.identifierBoston University [Boston] [BU]
hal.structure.identifierDepartment of Biomedical Engineering [Boston]
dc.contributor.authorDENNIS, Allison M.
dc.date.accessioned2020
dc.date.available2020
dc.date.issued2019
dc.date.conference2019
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/19696
dc.description.abstractEnAn alternative molecular recognition approach was developed for sensing small molecule analytes using the differential binding of an allosteric transcription factor (TF, specifically TetR) to its cognate DNA as the molecular recognition element coupled with fluorescent resonance energy transfer (FRET) to yield an internally calibrated optical signal transduction mechanism. Sensors were evaluated comprising Cy5-modified DNA (FRET acceptor) with either a tdTomato-TetR fusion protein (FP-TF) or quantum dot-TetR conjugate (QD-TF) as the FRET donor by measuring the ratio of acceptor and donor fluorescence intensities (FA/FD) with titrations of a derivative of the antibiotic tetracycline, anhydrous tetracycline (aTc). A proof-of-concept FRET-based biosensor was successfully demonstrated through the modulation of FA/FD signal intensities based on varying analyte concentrations. Sensor design parameters affecting overall signal-to-noise ratio and sensitivity of the sensors are also identified.
dc.language.isoen
dc.publisherSPIE
dc.title.enQD-FRET-based biosensing of small molecule analytes using transcription factor-DNA binding
dc.typeCommunication dans un congrès avec actes
dc.identifier.doi10.1117/12.2516576
dc.subject.halChimie/Polymères
dc.subject.halChimie/Matériaux
dc.subject.halPhysique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft]
dc.description.sponsorshipEuropeA Wearable Sensor for Hormones Based on a Native Microbial Sensing
bordeaux.page25
bordeaux.volume10892
bordeaux.hal.laboratoriesLaboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629*
bordeaux.institutionBordeaux INP
bordeaux.institutionUniversité de Bordeaux
bordeaux.countryUS
bordeaux.title.proceedingColloidal Nanoparticles for Biomedical Applications XIV
bordeaux.conference.citySan Francisco
bordeaux.peerReviewedoui
hal.identifierhal-02534212
hal.version1
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-02534212v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2019&rft.volume=10892&rft.spage=25&rft.epage=25&rft.au=NGUYEN,%20Thuy%20T.&CHERN,%20Margaret&BAER,%20R.%20C.&FAN,%20Andy&GRAZON,%20Chlo%C3%A9&rft.genre=proceeding


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