QD-FRET-based biosensing of small molecule analytes using transcription factor-DNA binding
dc.rights.license | open | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Department of Biomedical Engineering [Boston] | |
dc.contributor.author | NGUYEN, Thuy T. | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Department of Biomedical Engineering [Boston] | |
dc.contributor.author | CHERN, Margaret | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | National Emerging Infectious Diseases Laboratories [NEIDL] | |
dc.contributor.author | BAER, R. C. | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Department of Biomedical Engineering [Boston] | |
dc.contributor.author | FAN, Andy | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Department of Chemistry, Boston University | |
hal.structure.identifier | Laboratoire de Chimie des Polymères Organiques [LCPO] | |
hal.structure.identifier | Team 3 LCPO : Polymer Self-Assembly & Life Sciences | |
dc.contributor.author | GRAZON, Chloé | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Department of Biomedical Engineering [Boston] | |
dc.contributor.author | GALAGAN, James | |
hal.structure.identifier | Boston University [Boston] [BU] | |
hal.structure.identifier | Department of Biomedical Engineering [Boston] | |
dc.contributor.author | DENNIS, Allison M. | |
dc.date.accessioned | 2020 | |
dc.date.available | 2020 | |
dc.date.issued | 2019 | |
dc.date.conference | 2019 | |
dc.identifier.uri | https://oskar-bordeaux.fr/handle/20.500.12278/19696 | |
dc.description.abstractEn | An alternative molecular recognition approach was developed for sensing small molecule analytes using the differential binding of an allosteric transcription factor (TF, specifically TetR) to its cognate DNA as the molecular recognition element coupled with fluorescent resonance energy transfer (FRET) to yield an internally calibrated optical signal transduction mechanism. Sensors were evaluated comprising Cy5-modified DNA (FRET acceptor) with either a tdTomato-TetR fusion protein (FP-TF) or quantum dot-TetR conjugate (QD-TF) as the FRET donor by measuring the ratio of acceptor and donor fluorescence intensities (FA/FD) with titrations of a derivative of the antibiotic tetracycline, anhydrous tetracycline (aTc). A proof-of-concept FRET-based biosensor was successfully demonstrated through the modulation of FA/FD signal intensities based on varying analyte concentrations. Sensor design parameters affecting overall signal-to-noise ratio and sensitivity of the sensors are also identified. | |
dc.language.iso | en | |
dc.publisher | SPIE | |
dc.title.en | QD-FRET-based biosensing of small molecule analytes using transcription factor-DNA binding | |
dc.type | Communication dans un congrès avec actes | |
dc.identifier.doi | 10.1117/12.2516576 | |
dc.subject.hal | Chimie/Polymères | |
dc.subject.hal | Chimie/Matériaux | |
dc.subject.hal | Physique [physics]/Matière Condensée [cond-mat]/Matière Molle [cond-mat.soft] | |
dc.description.sponsorshipEurope | A Wearable Sensor for Hormones Based on a Native Microbial Sensing | |
bordeaux.page | 25 | |
bordeaux.volume | 10892 | |
bordeaux.hal.laboratories | Laboratoire de Chimie des Polymères Organiques (LCPO) - UMR 5629 | * |
bordeaux.institution | Bordeaux INP | |
bordeaux.institution | Université de Bordeaux | |
bordeaux.country | US | |
bordeaux.title.proceeding | Colloidal Nanoparticles for Biomedical Applications XIV | |
bordeaux.conference.city | San Francisco | |
bordeaux.peerReviewed | oui | |
hal.identifier | hal-02534212 | |
hal.version | 1 | |
hal.origin.link | https://hal.archives-ouvertes.fr//hal-02534212v1 | |
bordeaux.COinS | ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.date=2019&rft.volume=10892&rft.spage=25&rft.epage=25&rft.au=NGUYEN,%20Thuy%20T.&CHERN,%20Margaret&BAER,%20R.%20C.&FAN,%20Andy&GRAZON,%20Chlo%C3%A9&rft.genre=proceeding |
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