QD-FRET-based biosensing of small molecule analytes using transcription factor-DNA binding
BAER, R. C.
Boston University [Boston] [BU]
National Emerging Infectious Diseases Laboratories [NEIDL]
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Boston University [Boston] [BU]
National Emerging Infectious Diseases Laboratories [NEIDL]
BAER, R. C.
Boston University [Boston] [BU]
National Emerging Infectious Diseases Laboratories [NEIDL]
Boston University [Boston] [BU]
National Emerging Infectious Diseases Laboratories [NEIDL]
GRAZON, Chloé
Boston University [Boston] [BU]
Department of Chemistry, Boston University
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
< Reduce
Boston University [Boston] [BU]
Department of Chemistry, Boston University
Laboratoire de Chimie des Polymères Organiques [LCPO]
Team 3 LCPO : Polymer Self-Assembly & Life Sciences
Language
en
Communication dans un congrès avec actes
This item was published in
Colloidal Nanoparticles for Biomedical Applications XIV, 2019, San Francisco. 2019, vol. 10892, p. 25
SPIE
English Abstract
An alternative molecular recognition approach was developed for sensing small molecule analytes using the differential binding of an allosteric transcription factor (TF, specifically TetR) to its cognate DNA as the molecular ...Read more >
An alternative molecular recognition approach was developed for sensing small molecule analytes using the differential binding of an allosteric transcription factor (TF, specifically TetR) to its cognate DNA as the molecular recognition element coupled with fluorescent resonance energy transfer (FRET) to yield an internally calibrated optical signal transduction mechanism. Sensors were evaluated comprising Cy5-modified DNA (FRET acceptor) with either a tdTomato-TetR fusion protein (FP-TF) or quantum dot-TetR conjugate (QD-TF) as the FRET donor by measuring the ratio of acceptor and donor fluorescence intensities (FA/FD) with titrations of a derivative of the antibiotic tetracycline, anhydrous tetracycline (aTc). A proof-of-concept FRET-based biosensor was successfully demonstrated through the modulation of FA/FD signal intensities based on varying analyte concentrations. Sensor design parameters affecting overall signal-to-noise ratio and sensitivity of the sensors are also identified.Read less <
European Project
A Wearable Sensor for Hormones Based on a Native Microbial Sensing
Origin
Hal imported