VIGNET, Caroline; LARCHER, Thibaut; DAVAIL, Blandine; JOASSARD, Lucette; LE MENACH, Karyn; GUIONNET, Tiphaine; LYPHOUT, Laura; LEDEVIN, Mireille; GOUBEAU, Manon; BUDZINSKI, Hélène; BÉGOUT, Marie-Laure; COUSIN, Xavier

doi:10.3390/toxics4040026

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VIGNET, Caroline
Unité Biogéochimie et Ecotoxicologie [BE]

LARCHER, Thibaut
Physiopathologie Animale et bioThérapie du muscle et du système nerveux [PAnTher]

DAVAIL, Blandine

Environnements et Paléoenvironnements OCéaniques [EPOC]

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Article de revue

Ce document a été publié dans

Toxics. 2016-10-28, vol. 4, n° 4, p. 26

MDPI

Résumé en anglais

Polycyclic aromatic hydrocarbons (PAHs) constitute a large family of organic pollutants emitted in the environment as complex mixtures, the compositions of which depend on origin. Among a wide range of physiological defects, PAHs are suspected to be involved in disruption of reproduction. In an aquatic environment, the trophic route is an important source of chronic exposure to PAHs. Here, we performed trophic exposure of zebrafish to three fractions of different origin, one pyrolytic and two petrogenic. Produced diets contained PAHs at environmental concentrations. Reproductive traits were analyzed at individual, tissue and molecular levels. Reproductive success and cumulative eggs number were disrupted after exposure to all three fractions, albeit to various extents depending on the fraction and concentrations. Histological analyses revealed ovary maturation defects after exposure to all three fractions as well as degeneration after exposure to a pyrolytic fraction. In testis, hypoplasia was observed after exposure to petrogenic fractions. Genes expression analysis in gonads has allowed us to establish common pathways such as endocrine disruption or differentiation/maturation defects. Taken altogether, these results indicate that PAHs can indeed disrupt fish reproduction and that different fractions trigger different pathways resulting in different effects.< Réduire

Mots clés en anglais

molecular mechanisms

gonad differentiation

fertilization

spawning success

polycyclic aromatic hydrocarbons

zebrafish

URI

https://oskar-bordeaux.fr/handle/20.500.12278/194930

DOI

http://dx.doi.org/10.3390/toxics4040026

Project ANR

Contamination et physiologie des poissons aux polluants - ANR-09-CESA-0002

Unités de recherche

EPOC : Environnements et Paléoenvironnements Océaniques et Continentaux - UMR 5805