Afficher la notice abrégée

Identification of New Natural Sweet Compounds in Wine Using Centrifugal Partition Chromatography–Gustatometry and Fourier Transform Mass Spectrometry

dc.rights.licenseopenen_US
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorMARCHAL, Axel
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorWAFFO-TÉGUO, Pierre
dc.contributor.authorGENIN, Eric
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorMÉRILLON, Jean-Michel
hal.structure.identifierUnité de Recherche Œnologie [Villenave d'Ornon] [OENO]
dc.contributor.authorDUBOURDIEU, Denis
dc.date.accessioned2024-01-26T10:59:34Z
dc.date.available2024-01-26T10:59:34Z
dc.date.issued2011-12-15
dc.identifier.issn0003-2700en_US
dc.identifier.urioai:crossref.org:10.1021/ac202499a
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/187551
dc.description.abstractEnSweetness contributes notably to the taste-balance of dry wines and increases during oak-barrel aging owing to the release of natural sweeteners from wood. The search for such taste-active molecules, which are sometimes present at very low concentrations in wine or other complex matrixes, requires both reliable purification tools and powerful identification techniques. Here, we report the development of an original inductive method using centrifugal partition chromatography (CPC) and sensorial analysis. This method, called CPC_gustatometry, was implemented to isolate a sweet fraction with only four compounds from a complex oak wood extract. The recently developed Fourier transform mass spectrometry (FT-MS, Orbitrap analyzer) was used jointly with two-dimensional nuclear magnetic resonance (2D 1H and 13C NMR) to obtain the structural elucidation of the purified compounds. The tandem mass spectrometry (MS/MS) spectra obtained with resonant and nonresonant fragmentation modes were compared, thus providing complementary information about the molecular structure. Two oleanane-type triterpenoids substituted with galloyl and glucosyl moieties were identified, one of which exhibits sweet properties. We term these compounds which have never been reported, Quercotriterpenoside I and II.
dc.language.isoENen_US
dc.sourcecrossref
dc.title.enIdentification of New Natural Sweet Compounds in Wine Using Centrifugal Partition Chromatography–Gustatometry and Fourier Transform Mass Spectrometry
dc.typeArticle de revueen_US
dc.identifier.doi10.1021/ac202499aen_US
dc.subject.halSciences du Vivant [q-bio]/Biologie végétaleen_US
bordeaux.journalAnalytical Chemistryen_US
bordeaux.page9629-9637en_US
bordeaux.volume83en_US
bordeaux.hal.laboratoriesOenologie - UMR 1366en_US
bordeaux.issue24en_US
bordeaux.institutionUniversité de Bordeauxen_US
bordeaux.institutionBordeaux INPen_US
bordeaux.institutionINRAEen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcedissemin
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcedissemin
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Analytical%20Chemistry&rft.date=2011-12-15&rft.volume=83&rft.issue=24&rft.spage=9629-9637&rft.epage=9629-9637&rft.eissn=0003-2700&rft.issn=0003-2700&rft.au=MARCHAL,%20Axel&WAFFO-T%C3%89GUO,%20Pierre&GENIN,%20Eric&M%C3%89RILLON,%20Jean-Michel&DUBOURDIEU,%20Denis&rft.genre=article


Fichier(s) constituant ce document

Thumbnail
Nom:
OENO_AnalChem_2011_Marchal.pdf
Taille:
797.8Ko
Format:
PDF
Voir/Ouvrir

Ce document figure dans la(les) collection(s) suivante(s)

Afficher la notice abrégée