Developmental expression of ectonucleotidase and purinergic receptors detection by whole-mount in situ hybridization in Xenopus embryos
BLANCHARD, Camille
Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]
Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]
BLANCHARD, Camille
Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]
< Reduce
Neurocentre Magendie : Physiopathologie de la Plasticité Neuronale [U1215 Inserm - UB]
Language
EN
Article de revue
This item was published in
Methods in Molecular Biology. 2020, vol. 2041, p. 87-106
English Abstract
Xenopus embryos are one of the most used animal models in developmental biology and are well suited for apprehending functions of signaling pathways during embryogenesis. To do so, it is necessary to be able to detect ...Read more >
Xenopus embryos are one of the most used animal models in developmental biology and are well suited for apprehending functions of signaling pathways during embryogenesis. To do so, it is necessary to be able to detect expression pattern of the key genes of these signaling pathways. Here we describe the whole-mount in situ hybridization technique to investigate the expression pattern of ectonucleotidases and purinergic receptors during embryonic development. © Springer Science+Business Media, LLC, part of Springer Nature 2020.Read less <
English Keywords
Ectonucleotidases
Expression pattern
P2X receptors
Purinergic signaling pathway
Whole-mount in situ hybridization
Xenopus embryo