For over 30 years, the sources and the transfer dynamics of micropollutants have been investigated in the PIREN-Seine programme. Recent works included a wide range of chemicals and biological contaminants of emerging concern (i.e. contaminants whose occurrence, fate and impact are scarcely documented). This chapter presents a brief overview of research recently conducted on contaminants as diverse as macro- and microplastics, poly- and perfluoroalkyl substances (PFASs), pathogenic protozoa, antibiotics and the associated antibiotic resistance. The multiscalar study of plastics and PFASs at a large spatial scale is rare; the results produced in recent years on the Seine River catchment have provided an original contribution to the investigation of the dynamics of these contaminants in urban environments. The results also highlighted that pathogenic protozoa are ubiquitous in the Seine River basin and that the contamination of bivalves such as Dreissena polymorpha could reflect the ambient biological contamination of watercourses. The widespread occurrence of antibiotics in the Seine River was demonstrated, and it was shown that the resistome of biofilms in highly urbanised rivers constitutes a microenvironment where genetic support for antibiotic resistance (clinical integrons) and resistance genes for trace metals are concentrated. © 2020, The Author(s).Read less <