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Enzyme‐based kinetic modelling of ASC–GSH cycle during tomato fruit development reveals the importance of reducing power and ROS availability

hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDECROS, Guillaume
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDUSSARRAT, Thomas
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBALDET, Pierre
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorCASSAN, Cédric
hal.structure.identifierPlateforme Bordeaux Metabolome
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorCABASSON, Cécile
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDIEUAIDE‐NOUBHANI, Martine
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorDESTAILLEUR, Alice
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorFLANDIN, Amélie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorPRIGENT, Sylvain
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorMORI, Kentaro
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorCOLOMBIE, Sophie
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorJORLY, Joana
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorGIBON, Yves
hal.structure.identifierBiologie du fruit et pathologie [BFP]
dc.contributor.authorBEAUVOIT, Bertrand
hal.structure.identifierBiologie du fruit et pathologie [BFP]
hal.structure.identifierPlateforme Bordeaux Metabolome
dc.contributor.authorPÉTRIACQ, Pierre
dc.date.issued2023-08-07
dc.identifier.issn0028-646X
dc.description.abstractEnSummary The ascorbate–glutathione (ASC–GSH) cycle is at the heart of redox metabolism, linking the major redox buffers with central metabolism through the processing of reactive oxygen species (ROS) and pyridine nucleotide metabolism. Tomato fruit development is underpinned by changes in redox buffer contents and their associated enzyme capacities, but interactions between them remain unclear. Based on quantitative data obtained for the core redox metabolism, we built an enzyme‐based kinetic model to calculate redox metabolite concentrations with their corresponding fluxes and control coefficients. Dynamic and associated regulations of the ASC–GSH cycle throughout the whole fruit development were analysed and pointed to a sequential metabolic control of redox fluxes by ASC synthesis, NAD(P)H and ROS availability depending on the developmental phase. Furthermore, we highlighted that monodehydroascorbate reductase and the availability of reducing power were found to be the main regulators of the redox state of ASC and GSH during fruit growth under optimal conditions. Our kinetic modelling approach indicated that tomato fruit development displayed growth phase‐dependent redox metabolism linked with central metabolism via pyridine nucleotides and H 2 O 2 availability, while providing a new tool to the scientific community to investigate redox metabolism in fruits.
dc.description.sponsorshipModélisation intégrative du fruit pour un système de sélection unifié
dc.description.sponsorshipDéveloppement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation - ANR-11-INBS-0010
dc.description.sponsorshipCentre français de phénomique végétale - ANR-11-INBS-0012
dc.language.isoen
dc.publisherWiley
dc.rights.urihttp://creativecommons.org/licenses/by-nc/
dc.subject.enASC-GSH cycle
dc.subject.enenzyme-based kinetic modelling
dc.subject.enfruit development
dc.subject.enmetabolism
dc.subject.enredox
dc.subject.entomato
dc.title.enEnzyme‐based kinetic modelling of ASC–GSH cycle during tomato fruit development reveals the importance of reducing power and ROS availability
dc.typeArticle de revue
dc.identifier.doi10.1111/nph.19160
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.description.sponsorshipEuropeEuropean Commission’s Horizon 2020 Research and Innovation program via the GLOMICAVE project under grant agreement no. 952908
bordeaux.journalNew Phytologist
bordeaux.peerReviewedoui
hal.identifierhal-04178513
hal.version1
hal.popularnon
hal.audienceInternationale
hal.origin.linkhttps://hal.archives-ouvertes.fr//hal-04178513v1
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=New%20Phytologist&rft.date=2023-08-07&rft.eissn=0028-646X&rft.issn=0028-646X&rft.au=DECROS,%20Guillaume&DUSSARRAT,%20Thomas&BALDET,%20Pierre&CASSAN,%20C%C3%A9dric&CABASSON,%20C%C3%A9cile&rft.genre=article


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