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Exploring the impact of the side-chain length on peptide/RNA binding events.
Language
EN
Article de revue
This item was published in
Physical Chemistry Chemical Physics. 2017-07-19, vol. 19, n° 28, p. 18452-18460
English Abstract
The impact of the amino-acid side-chain length on peptide-RNA binding events has been investigated using HIV-1 Tat derived peptides as ligands and the HIV-1 TAR RNA element as an RNA model. Our studies demonstrate that ...Read more >
The impact of the amino-acid side-chain length on peptide-RNA binding events has been investigated using HIV-1 Tat derived peptides as ligands and the HIV-1 TAR RNA element as an RNA model. Our studies demonstrate that increasing the length of all peptide side-chains improves unexpectedly the binding affinity (K) but reduces the degree of compactness of the peptide-RNA complex. Overall, the side-chain length appears to modulate in an unpredictable way the ability of the peptide to compete with the cognate TAR RNA partner. Beyond the establishment of non-intuitive fundamental relationships, our results open up new perspectives in the design of effective RNA ligand competitors, since a large number of them have already been identified but few studies report on the modulation of the biological activity by modifying in the same way the length of all chains connecting RNA recognition motives to the central scaffold of a ligand.Read less <
English Keywords
Amino Acid Sequence
HIV Long Terminal Repeat
HIV-1
Humans
Molecular Dynamics Simulation
Peptides
Phase Transition
Protein Binding
RNA
Viral
Spectrophotometry
Ultraviolet
Spectroscopy
Fourier Transform Infrared
Temperature
Thermodynamics
Ultraviolet Rays