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Changes in adenoviral chromatin organization precede early gene activation upon infection.

dc.rights.licenseopenen_US
dc.contributor.authorSCHWARTZ, Uwe
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorKOMATSU, Tetsuro
dc.contributor.authorHUBER, Claudia
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorLAGADEC, Floriane
dc.contributor.authorBAUMGARTL, Conradin
dc.contributor.authorSILBERHORN, Elisabeth
dc.contributor.authorNUETZEL, Margit
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorRAYNE, Fabienne
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBASYUK, Eugenia
dc.contributor.authorBERTRAND, Edouard
dc.contributor.authorREHLI, Michael
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorWODRICH, Harald
dc.contributor.authorLAENGST, Gernot
dc.date.accessioned2023-11-03T15:27:03Z
dc.date.available2023-11-03T15:27:03Z
dc.date.issued2023-10-04
dc.identifier.issn1460-2075en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/184590
dc.description.abstractEnWithin the virion, adenovirus DNA associates with the virus-encoded, protamine-like structural protein pVII. Whether this association is organized, and how genome packaging changes during infection and subsequent transcriptional activation is currently unclear. Here, we combined RNA-seq, MNase-seq, ChIP-seq, and single genome imaging during early adenovirus infection to unveil the structure- and time-resolved dynamics of viral chromatin changes as well as their correlation with gene transcription. Our MNase mapping data indicates that the adenoviral genome is arranged in precisely positioned nucleoprotein particles with nucleosome-like characteristics, that we term adenosomes. We identified 238 adenosomes that are positioned by a DNA sequence code and protect about 60-70 bp of DNA. The incoming adenoviral genome is more accessible at early gene loci that undergo additional chromatin de-condensation upon infection. Histone H3.3 containing nucleosomes specifically replaces pVII at distinct genomic sites and at the transcription start sites of early genes. Acetylation of H3.3 is predominant at the transcription start sites and precedes transcriptional activation. Based on our results, we propose a central role for the viral pVII nucleoprotein architecture, which is required for the dynamic structural changes during early infection, including the regulation of nucleosome assembly prior to transcription initiation. Our study thus may aid the rational development of recombinant adenoviral vectors exhibiting sustained expression in gene therapy.
dc.language.isoENen_US
dc.subject.enNucleosomes
dc.subject.enTranscriptional Activation
dc.subject.enChromatin
dc.subject.enDNA
dc.subject.enChromatin Assembly and Disassembly
dc.subject.enAdenoviridae
dc.title.enChanges in adenoviral chromatin organization precede early gene activation upon infection.
dc.title.alternativeEMBO Jen_US
dc.typeArticle de revueen_US
dc.identifier.doi10.15252/embj.2023114162en_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologieen_US
dc.identifier.pubmed37641864en_US
bordeaux.journalEMBO Journalen_US
bordeaux.pagee114162en_US
bordeaux.volume42en_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.issue19en_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcepubmed
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcepubmed
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=EMBO%20Journal&rft.date=2023-10-04&rft.volume=42&rft.issue=19&rft.spage=e114162&rft.epage=e114162&rft.eissn=1460-2075&rft.issn=1460-2075&rft.au=SCHWARTZ,%20Uwe&KOMATSU,%20Tetsuro&HUBER,%20Claudia&LAGADEC,%20Floriane&BAUMGARTL,%20Conradin&rft.genre=article


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