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Micafungin susceptibility of the most common Candida species in 16 French university hospitals : comparison between the Etest® and the EUCAST methods (MICACAND study)

dc.contributor.authorBOUGNOUX, Marie-Elisabeth
dc.contributor.authorDANNAOUI, Eric
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorACCOCEBERRY, Isabelle
IDREF: 075520818
dc.contributor.authorANGOULEVANT, Adela
dc.contributor.authorBAILLY, E.
dc.contributor.authorBOUCHARA, Jean-Philippe
dc.contributor.authorBOTTEREL, Françoise
dc.contributor.authorCHEVRIER, Sylviane
dc.contributor.authorCHOUAKI, Taieb
dc.contributor.authorCORNET, Muriel
dc.contributor.authorFEKKAR, Arnault
dc.contributor.authorDALLE, Frédéric
dc.contributor.authorGANGNEUX, Jean-Pierre
dc.contributor.authorGUITARD, Juliette
dc.contributor.authorHENNEQUIN, Christophe
dc.contributor.authorLEPAPE, Patrice
dc.contributor.authorMAUBON, Danièle
dc.contributor.authorRANQUE, Stéphane
dc.contributor.authorSENDID, Boualem
dc.contributor.authorCHANDENIER, Jacques
dc.date.accessioned2023-07-18T08:52:10Z
dc.date.available2023-07-18T08:52:10Z
dc.date.conference2015-04-25
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/183426
dc.description.abstractEnObjectives : Micafungin is currently used in France. The aim of this study is to determine its activity against a recent (2014) French collection of Candida isolates. Although EUCAST is the reference method for in vitro antifungal susceptibility testing, it is not commonly used in routine clinical microbiology laboratories. Thus, it is important to evaluate alternative methods. We compared EUCAST and Etest for micafungin susceptibility testing of Candida spp. and we monitored the emergence of resistance. Methods: Sixteen centers (6 in Paris area and 10 across France) participated in a two-months prospective study. Clinical isolates of various Candida species (mainly C. albicans, C. glabrata, C. tropicalis, C. parapsilosis, C. kefyr and C. krusei, about 10 isolates of each species per center) were tested by Etest, according to manufacturer’s instructions. All isolates were subsequently centralized in one center for MIC determination by EUCAST method. For comparison purposes, Etest MICs were raised to the next higher EUCAST concentration. Resistance was defined based on EUCAST clinical breakpoints or on epidemiological cut-off values when clinical breakpoints were not available. Results : A total number of 933 Candida isolates were tested. The overall agreement (+/- 2 log2 dilutions) between EUCAST and Etest was 97.9%. Species n E-Test EUCAST MIC range MIC range % agreement with Etest % resistance C. albicans 159 ≤ 0.015 - 0.06 ≤ 0.015 - 0.06 100 1.3 C. tropicalis 152 ≤ 0.015 - 0.5 ≤ 0.015 - 1 98.7 0.7 C. parapsilosis 152 ≤ 0.015 - 4 ≤ 0.125 - 4 96.1 1.3 C. glabrata 152 ≤ 0.015 - 0.125 ≤ 0.015 - 1 98.7 3.9 C. kefyr 136 ≤ 0.015 - 0.25 ≤ 0.015 - 0.125 97.8 ND C. krusei 127 ≤ 0.015 - 1 ≤ 0.015 - 0.25 96.9 0 Other Candida species* 55 ≤ 0.015 - 1 ≤ 0.015 - 1 94.5 ND Total 933 ≤ 0.015 - 4 ≤ 0.015 - 4 97.9 ND *: C. lusitaniae, C. guilliermondii, C. norvegensis, C. inconspicua, C. famata, C. pelliculosa, C. lambica, C. sphaerica, C. ciferii, C. catenulata, C. utilis, C. colliculosa, C. nivariensis Conclusions : This study demonstrated a very good agreement between Etest, performed on a routine basis, and EUCAST for micafungin MIC determination. Micafungin resistance among the main Candida species was uncommon.
dc.language.isoen
dc.subjectE-test
dc.subject.enMicafungin
dc.subject.enCandida
dc.title.enMicafungin susceptibility of the most common Candida species in 16 French university hospitals : comparison between the Etest® and the EUCAST methods (MICACAND study)
dc.typePosteren_US
dc.subject.halSciences du Vivant [q-bio]
dc.subject.halSciences du Vivant [q-bio]/Biologie végétale
dc.subject.halSciences de l'environnement
bordeaux.page332 p.
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.institutionCNRS
bordeaux.countryDK
bordeaux.conference.cityCopenhague
bordeaux.peerReviewedoui
bordeaux.import.sourcehal
hal.identifierhal-02743560
hal.version1
hal.invitednonen_US
hal.conference.organizerEuropean Congress of Clinical Microbiology and Infectious Deseases (ECCMID). INT.en_US
hal.conference.end2015-04-28
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.spage=332%20p.&rft.epage=332%20p.&rft.au=BOUGNOUX,%20Marie-Elisabeth&DANNAOUI,%20Eric&ACCOCEBERRY,%20Isabelle&ANGOULEVANT,%20Adela&BAILLY,%20E.&rft.genre=unknown


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