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Unmasking the ancestral activity of integron integrases reveals a smooth evolutionary transition during functional innovation
Language
en
Article de revue
This item was published in
2016-03-10, vol. 7, p. Article number 10937
Nature Publishing Group
English Abstract
Tyrosine (Y)-recombinases have evolved to deliver mechanistically different reactions on a variety of substrates, but these evolutionary transitions are poorly understood. Among them, integron integrases are hybrid systems ...Read more >
Tyrosine (Y)-recombinases have evolved to deliver mechanistically different reactions on a variety of substrates, but these evolutionary transitions are poorly understood. Among them, integron integrases are hybrid systems recombining single-and double-stranded DNA partners. These reactions are asymmetric and need a replicative resolution pathway, an exception to the canonical second strand exchange model of Y-recombinases. Integron integrases possess a specific domain for this specialized pathway. Here we show that despite this, integrases are still capable of efficiently operating the ancestral second strand exchange in symmetrical reactions between double-stranded substrates. During these reactions, both strands are reactive and Holliday junction resolution can follow either pathway. A novel deep-sequencing approach allows mapping of the crossover point for the second strand exchange. The persistence of the ancestral activity in integrases illustrates their robustness and shows that innovation towards new recombination substrates and resolution pathways was a smooth evolutionary process.Read less <
English Keywords
Biological sciences
Molecular biology
European Project
Evolution and Transfer of Antibiotic Resistance
The Integron Cassette Dynamics and the Integrase Gene Expression
The Integron Cassette Dynamics and the Integrase Gene Expression
ANR Project
Integrative Biology of Emerging Infectious Diseases - ANR-10-LABX-0062
Recombination des cassettes d'intégron: dynamique in vivo et in vitro - ANR-12-BSV3-0015
Recombination des cassettes d'intégron: dynamique in vivo et in vitro - ANR-12-BSV3-0015