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hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorRAYNE, Fabienne
dc.contributor.authorDEBAISIEUX, Solène
dc.contributor.authorTU, Annie
dc.contributor.authorCHOPARD, Christophe
dc.contributor.authorTRYOEN-TOTH, Petra
dc.contributor.authorBEAUMELLE, Bruno
dc.date.accessioned2023-07-04T11:52:33Z
dc.date.available2023-07-04T11:52:33Z
dc.date.issued2016
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/183265
dc.description.abstractEnHIV-1 Tat is efficiently secreted by HIV-1-infected or Tat-transfected cells. Accordingly, Tat concentrations in the nanomolar range have been measured in the sera of HIV-1-infected patients, and this protein acts as a viral toxin on bystander cells. Nevertheless, assaying Tat concentration in media or sera is not that straightforward because extracellular Tat is unstable and particularly sensitive to oxidation. Moreover, most anti-Tat antibodies display limited affinity. Here, we describe methods to quantify extracellular Tat using a sandwich ELISA or Western blotting when Tat is secreted by suspension or adherent cells, respectively. In both cases it is important to capture exported Tat using antibodies before any Tat oxidation occurs; otherwise it will become denatured and unreactive toward antibodies.
dc.language.isoen
dc.source.titleHIV ProtocolsThird editionen_US
dc.title.enDetecting HIV-1 Tat in Cell Culture Supernatants by ELISA or Western Blot
dc.typeChapitre d'ouvrageen_US
dc.subject.halSciences du Vivant [q-bio]/Biologie cellulaire
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
bordeaux.page329-342
bordeaux.volume1354
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.institutionCNRS
bordeaux.import.sourcehal
hal.identifierhal-02137760
hal.version1
hal.popularnonen_US
hal.audienceInternationaleen_US
hal.exportfalse
workflow.import.sourcehal
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.btitle=HIV%20ProtocolsThird%20edition&rft.date=2016&rft.volume=1354&rft.spage=329-342&rft.epage=329-342&rft.au=RAYNE,%20Fabienne&DEBAISIEUX,%20Sol%C3%A8ne&TU,%20Annie&CHOPARD,%20Christophe&TRYOEN-TOTH,%20Petra&rft.genre=unknown


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