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Modulation of chromatin structure by the FACT histone chaperone complex regulates HIV-1 integration

dc.rights.licenseopenen_US
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorMATYSIAK, Julien
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorLESBATS, Paul
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorMAURO, Eric
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorLAPAILLERIE, Delphine
hal.structure.identifierCentre Génomique Fonctionnelle Bordeaux [Bordeaux] [CGFB]
dc.contributor.authorDUPUY, Jean-William
dc.contributor.authorLOPEZ, Angelica
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorBENLEULMI, Mohamed
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorCALMELS, Christina
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorANDREOLA, Marie-Line
dc.contributor.authorRUFF, Marc
dc.contributor.authorLLANO, Manuel
dc.contributor.authorDELELIS, Olivier
dc.contributor.authorLAVIGNE, Marc
hal.structure.identifierMicrobiologie Fondamentale et Pathogénicité [MFP]
dc.contributor.authorPARISSI, Vincent
dc.date.accessioned2023-06-28T10:23:22Z
dc.date.available2023-06-28T10:23:22Z
dc.date.issued2017-07-28
dc.identifier.issn1742-4690en_US
dc.identifier.urihttps://oskar-bordeaux.fr/handle/20.500.12278/183218
dc.description.abstractEnBackground: Insertion of retroviral genome DNA occurs in the chromatin of the host cell. This step is modulated by chromatin structure as nucleosomes compaction was shown to prevent HIV-1 integration and chromatin remodeling has been reported to affect integration efficiency. LEDGF/p75-mediated targeting of the integration complex toward RNA polymerase II (polII) transcribed regions ensures optimal access to dynamic regions that are suitable for integration. Consequently, we have investigated the involvement of polII-associated factors in the regulation of HIV-1 integration.Results: Using a pull down approach coupled with mass spectrometry, we have selected the FACT (FAcilitates Chromatin Transcription) complex as a new potential cofactor of HIV-1 integration. FACT is a histone chaperone complex associated with the polII transcription machinery and recently shown to bind LEDGF/p75. We report here that a tripartite complex can be formed between HIV-1 integrase, LEDGF/p75 and FACT in vitro and in cells. Biochemical analyzes show that FACT-dependent nucleosome disassembly promotes HIV-1 integration into chromatinized templates, and generates highly favored nucleosomal structures in vitro. This effect was found to be amplified by LEDGF/p75. Promotion of this FACT-mediated chromatin remodeling in cells both increases chromatin accessibility and stimulates HIV-1 infectivity and integration.Conclusions: Altogether, our data indicate that FACT regulates HIV-1 integration by inducing local nucleosomes dissociation that modulates the functional association between the incoming intasome and the targeted nucleosome.
dc.description.sponsorshipContrôle cellulaire de la sélectivité de l'intégration rétrovirale - ANR-11-JSV3-0006en_US
dc.description.sponsorshipInfrastructure Française pour la Biologie Structurale Intégréeen_US
dc.language.isoENen_US
dc.rights.urihttp://creativecommons.org/licenses/by/
dc.subject.enRetroviral integration
dc.subject.enHIV-1
dc.subject.enIntegrase
dc.subject.enChromatin
dc.subject.enNucleosome
dc.subject.enFACT
dc.subject.meshCells, Cultured
dc.subject.meshChromatin
dc.subject.meshNucleosomes
dc.subject.meshProtein Binding
dc.subject.meshVirus Integration
dc.subject.meshChromatin Assembly and Disassembly
dc.subject.meshHIV Infections
dc.subject.meshHIV Integrase
dc.subject.meshHIV-1
dc.subject.meshHistone Chaperones
dc.subject.meshHost-Pathogen Interactions
dc.subject.meshHumans
dc.subject.meshIntercellular Signaling Peptides and Proteins
dc.title.enModulation of chromatin structure by the FACT histone chaperone complex regulates HIV-1 integration
dc.typeArticle de revueen_US
dc.identifier.doi10.1186/s12977-017-0363-4en_US
dc.subject.halSciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologieen_US
dc.subject.halSciences du Vivant [q-bio]/Médecine humaine et pathologie/Maladies infectieusesen_US
bordeaux.journalRetrovirologyen_US
bordeaux.page39en_US
bordeaux.volume14en_US
bordeaux.hal.laboratoriesMFP (Laboratoire Microbiologie Fondamentale et Pathogénicité) - UMR 5234en_US
bordeaux.issue1en_US
bordeaux.institutionCNRSen_US
bordeaux.peerReviewedouien_US
bordeaux.inpressnonen_US
bordeaux.import.sourcehal
hal.identifierhal-03531801
hal.version1
hal.exportfalse
workflow.import.sourcehal
dc.rights.ccPas de Licence CCen_US
bordeaux.COinSctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.jtitle=Retrovirology&rft.date=2017-07-28&rft.volume=14&rft.issue=1&rft.spage=39&rft.epage=39&rft.eissn=1742-4690&rft.issn=1742-4690&rft.au=MATYSIAK,%20Julien&LESBATS,%20Paul&MAURO,%20Eric&LAPAILLERIE,%20Delphine&DUPUY,%20Jean-William&rft.genre=article


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